Beyler S A, Zaneveld L J
Fertil Steril. 1979 Dec;32(6):671-5. doi: 10.1016/s0015-0282(16)44417-1.
Enzymes have been implicated in facilitating cervical mucus penetration by spermatozoa. One of these enzymes in the neutral proteinase acrosin, which is associated with the sperm acrosome. To determine the validity of this hypothesis, human spermatozoa were incubated with the following acrosin inhibitors: p-aminobenzamidine (AB), N-alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK), and p-nitropheyl-p'-guanidino benzoate (NPGB). An in vitro slide test system was developed which allowed inhibitor-treated and control spermatozoa to be evaluated against the same human cervical mucus sample. At inhibitor concentrations far exceeding those necessary for the inhibition of human acrosin, there was no effect on spermatozoal penetration into or through the mucus. These findings indicate that, in man, acrosin activity is neither necessary nor facilitory to sperm penetration of cervical mucus. Evidence is also presented that demonstrates the superiority of the newly developed double-interface slide test, especially for comparative purposes, over the tests currently in use.
酶被认为在促进精子穿透宫颈黏液方面发挥作用。其中一种酶是中性蛋白酶顶体酶，它与精子顶体相关。为了确定这一假设的正确性，将人类精子与以下顶体酶抑制剂一起孵育：对氨基苯甲脒（AB）、N-α-对甲苯磺酰-L-赖氨酸氯甲基酮（TLCK）和对硝基苯基-p'-胍基苯甲酸酯（NPGB）。开发了一种体外玻片测试系统，该系统可以针对同一份人类宫颈黏液样本对经抑制剂处理的精子和对照精子进行评估。在抑制剂浓度远远超过抑制人类顶体酶所需浓度的情况下，对精子穿透黏液或穿过黏液没有影响。这些发现表明，在人类中，顶体酶活性对于精子穿透宫颈黏液既不是必需的，也没有促进作用。还提供了证据，证明新开发的双界面玻片测试，特别是用于比较目的时，优于目前使用的测试。
