Key Laboratory for Biorheological Science and Technology of Ministry of Education, College of Chongqing University, Chongqing, 400044, PR China.
Key Laboratory for Biorheological Science and Technology of Ministry of Education, College of Chongqing University, Chongqing, 400044, PR China.
Talanta. 2024 Oct 1;278:126481. doi: 10.1016/j.talanta.2024.126481. Epub 2024 Jun 26.
Quantitative microRNA (miRNA) detection is crucial for early breast cancer diagnosis and prognosis. However, quick and stable fluorescence sensing for miRNA identification is still challenging. This work developed a novel label-free detection method based on AuNPs etching for quantitatively detecting miRNA-155. A layer of AuNPs was grown on the surface of mesoporous silica nanoparticles (MSN) loaded with Rhodamine 6G (R6G) using seed-mediated growth, followed by probe attachment. In the presence of miRNA-155, the MSN@R6G@AuNP surface loses the protection of the attached probe, rendering AuNPs susceptible to etching by hydrochloric acid. This results in a significant fluorescent signal being released in the free space. The encapsulation with AuNPs effectively reduces signal leakage, while the rapid etching process shortens detection time. This strategy enables sensitive and fast detection with a detection range of 100 fM to 100 nM, a detection limit of 2.18 fM, and a detection time of 30 min. The recovery rate in normal human serum ranges from 99.02 % to 106.34 %. This work presents a simple biosensing strategy with significant potential for application in tumor diagnosis.
定量检测 microRNA（miRNA）对于早期乳腺癌的诊断和预后至关重要。然而，快速且稳定的荧光传感方法对于 miRNA 的识别仍然具有挑战性。本工作开发了一种基于金纳米颗粒（AuNPs）刻蚀的新型无标记检测方法，用于定量检测 miRNA-155。通过种子介导生长在负载 Rhodamine 6G（R6G）的介孔硅纳米颗粒（MSN）表面生长一层 AuNPs，然后再连接探针。在 miRNA-155 的存在下，MSN@R6G@AuNP 表面失去了附着探针的保护，使得 AuNPs 容易被盐酸刻蚀。这导致在自由空间中释放出显著的荧光信号。AuNPs 的封装有效地减少了信号泄漏，而快速的刻蚀过程缩短了检测时间。该策略实现了灵敏快速的检测，检测范围为 100 fM 至 100 nM，检测限为 2.18 fM，检测时间为 30 分钟。在正常人血清中的回收率范围为 99.02%至 106.34%。本工作提出了一种简单的生物传感策略，具有在肿瘤诊断中应用的巨大潜力。
