Department of Analytical Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), 13083-970, Brazil; National Institute of Science and Technology in Bioanalytic (INCTBio), Brazil.
Department of Analytical Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), 13083-970, Brazil; National Institute of Science and Technology in Bioanalytic (INCTBio), Brazil.
Talanta. 2024 Oct 1;278:126467. doi: 10.1016/j.talanta.2024.126467. Epub 2024 Jun 28.
The combination of CRISPR technology and electrochemical sensors has sparked a paradigm shift in the landscape of point-of-care (POC) diagnostics. This review explores the dynamic convergence between CRISPR and electrochemical sensing, elucidating their roles in rapid and precise biosensing platforms. CRISPR, renowned for its remarkable precision in genome editing and programmability capability, has found a novel application in conjunction with electrochemical sensors, promising highly sensitive and specific detection of nucleic acids and biomarkers associated with diverse diseases. This article navigates through fundamental principles, research developments, and applications of CRISPR-based electrochemical sensors, highlighting their potential to revolutionize healthcare accessibility and patient outcomes. In addition, some key points and challenges regarding applying CRISPR-powered electrochemical sensors in real POC settings are presented. By discussing recent advancements and challenges in this interdisciplinary field, this review evaluates the potential of these innovative sensors as an alternative for decentralized, rapid, and accurate POC testing, offering some insights into their applications across clinical scenarios and their impact on the future of diagnostics.
CRISPR 技术与电化学传感器的结合，正在引发即时诊断（POC）领域的范式转变。本综述探讨了 CRISPR 与电化学传感之间的动态融合，阐明了它们在快速、精确生物传感平台中的作用。CRISPR 以其在基因组编辑方面的出色精度和可编程性而闻名，现已与电化学传感器相结合，有望实现与各种疾病相关的核酸和生物标志物的高灵敏度和特异性检测。本文通过导航基本原理、研究进展和基于 CRISPR 的电化学传感器的应用，突出了它们在改善医疗保健可及性和患者结果方面的潜力。此外，还介绍了在实际 POC 环境中应用基于 CRISPR 的电化学传感器的一些关键点和挑战。通过讨论这个跨学科领域的最新进展和挑战，本综述评估了这些创新传感器作为去中心化、快速和精确 POC 测试的替代方案的潜力，为它们在临床场景中的应用及其对诊断未来的影响提供了一些见解。
