PG and Research Department of Zoology, Bishop Heber College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620017, India.
Department of Zoology, Thanthai Periyar Government Arts and Science College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620017, India.
Mol Biol Rep. 2024 Jul 6;51(1):787. doi: 10.1007/s11033-024-09720-1.
A molecular approach for the identification of unknown species by the using mitochondrial cox1 gene is an effective and reliable as compared with morphological-based identification. Hirudinaria manillensis referred to as Asian Buffalo Leech, is found in South Asia and traditionally used as medicine owing to its medicinal properties.
The study aimed to isolate and identify the leech species using cox1 gene sequencing and their phylogenetic relationships. The nucleotide sequences of cytochrome c oxidase subunit I (cox1) mitochondrial genes were analyzed for species identification and the phylogenetic relationship of crucial therapeutic leech Hirudinaria manillensis. The isolated DNA from the leech sample was amplified with cox1 gene-specific primers. BLAST results with the H. manillensis sequence showed 89.24% homology with H. manillensis and phylogenetic tree analysis revealed the genetic relationship with other GenBank submitted sequences.
The present study concluded that the cox1 gene could be an effective way to identify the leech H. manillensis and provided sufficient phylogenetic information to distinguish H. manillensis indicating a significant mtDNA-based approach to species identification.
与基于形态的鉴定相比，利用线粒体 cox1 基因鉴定未知物种的分子方法更加有效和可靠。蛭类被称为亚洲水牛蛭，分布于南亚，由于其药用特性，传统上被用作药物。
本研究旨在通过 cox1 基因测序对蛭类进行分离和鉴定，并分析其系统发育关系。对细胞色素 c 氧化酶亚基 I (cox1) 线粒体基因的核苷酸序列进行分析，以确定关键治疗性蛭类 Hirudinaria manillensis 的物种鉴定和系统发育关系。从蛭类样本中提取的 DNA 用 cox1 基因特异性引物进行扩增。与 H. manillensis 序列的 BLAST 结果显示 89.24% 的同源性与 H. manillensis 一致，系统发育树分析显示与其他 GenBank 提交序列的遗传关系。
本研究表明 cox1 基因可有效鉴定 Hirudinaria manillensis，并提供了足够的系统发育信息来区分 Hirudinaria manillensis，表明基于 mtDNA 的物种鉴定方法具有重要意义。
