Grogan J B, Subba Rao D S, Henry S E
Invest Ophthalmol Vis Sci. 1985 Sep;26(9):1230-5.
This study shows that alloantigen presentation via the anterior chamber (AC) of the eye coupled with a single high dose cyclophosphamide (CP) (100 mg/kg) treatment effectively suppresses the skin graft rejection reaction of the recipient. Lewis (Le) rats bearing allogeneic Brown Norway (BN) skin implants in the AC of the eye demonstrate a modest increase in the survival time of orthotopic BN skin grafts. A slight prolongation of the survival of orthotopic BN skin grafts was also demonstrated in nonimplant or syngeneic implant-bearing Le recipients which received a single injection of a large dose of CP. Augmentation of suppression was evident in rats which were treated with a single dose of 75 mg/kg CP but not 25 mg/kg. The augmentation of suppression was evident when CP treatment and skin grafting of the recipient occurred on either 0, 7, or 14 days postimplantation. Recipient splenectomy did not interfere with the augmentation of suppression.
本研究表明，通过眼的前房（AC）呈递同种异体抗原并结合单次高剂量环磷酰胺（CP）（100mg/kg）治疗可有效抑制受体的皮肤移植排斥反应。在眼的前房中植入同种异体棕色挪威（BN）皮肤移植物的刘易斯（Le）大鼠，其原位BN皮肤移植物的存活时间适度延长。在接受单次大剂量CP注射的未植入或植入同基因移植物的Le受体中，也证实原位BN皮肤移植物的存活略有延长。在接受75mg/kg CP单剂量治疗而非25mg/kg治疗的大鼠中，抑制作用增强明显。当在植入后0、7或14天对受体进行CP治疗和皮肤移植时，抑制作用增强明显。受体脾切除术并不干扰抑制作用的增强。
