Hanyang University Institute for Rheumatology Research (HYIRR), Hanyang University, Seoul 04763, Republic of Korea.
Hanyang University Institute for Rheumatology Research (HYIRR), Hanyang University, Seoul 04763, Republic of Korea; Department of Rheumatology, Hanyang University Hospital for Rheumatic Diseases, Seoul 04763, Republic of Korea.
Life Sci. 2024 Sep 1;352:122894. doi: 10.1016/j.lfs.2024.122894. Epub 2024 Jul 4.
This study assessed the therapeutic potential of swimming exercise in the curdlan-injected SKG mouse model and investigated the modulatory effects of irisin on inflammation. Curdlan-injected SKG were randomly assigned to either a home-cage group or a swimming group for 6 weeks. Changes in clinical arthritis scores and ankle thickness were measured weekly. Post-swimming program, mice were anesthetized for collection of vastus lateralis muscle and blood, which was followed by histological analysis, micro-CT imaging of the ankle joints, and the measurement of pro-inflammatory cytokines and irisin levels. Additionally, curdlan-injected SKG mice were intravenously injected with recombinant irisin protein and observed. Finally, serum levels of irisin in healthy control and ankylosing spondylitis (AS) patient groups were measured by ELISA. The swimming group of curdlan-injected SKG mice exhibited significant improvements in arthritis and enthesitis compared to the home-cage group. In particular, micro-CT and histological analyses revealed a notable reduction in pathological bone features in the swimming group compared to the home-cage group. Muscle endurance was also enhanced in the swimming group compared to the home-cage group, as determined by the wire-hanging test. Intriguingly, irisin levels not only were statistically increased in the swimming group but, also, TNF-α, IL-1β, and IL-6 levels were decreased. Additionally, injection of irisin protein slightly attenuated both arthritis and enthesitis in curdlan-injected SKG mice. Meanwhile, irisin serum levels were declined in AS patients. Overall, we found that swimming exercise attenuated pathological bone features in an AS animal model, potentially mediated by increased irisin serum levels with associated anti-inflammatory effects.
本研究评估了游泳运动对琼脂糖聚糖诱导的 SKG 小鼠模型的治疗潜力，并研究了鸢尾素对炎症的调节作用。琼脂糖聚糖诱导的 SKG 被随机分为笼内组或游泳组，进行 6 周的游泳运动。每周测量临床关节炎评分和踝关节厚度的变化。游泳运动方案结束后，麻醉小鼠采集股外侧肌和血液，进行组织学分析、踝关节 micro-CT 成像以及促炎细胞因子和鸢尾素水平的测量。此外，还对琼脂糖聚糖诱导的 SKG 小鼠进行了重组鸢尾素蛋白的静脉注射观察。最后，通过 ELISA 测量健康对照组和强直性脊柱炎（AS）患者组的血清鸢尾素水平。与笼内组相比，琼脂糖聚糖诱导的 SKG 游泳组的关节炎和附着点炎均有明显改善。特别是与笼内组相比，游泳组的 micro-CT 和组织学分析显示病理性骨特征明显减少。与笼内组相比，游泳组的肌肉耐力也有所提高，这是通过悬线试验确定的。有趣的是，游泳组的鸢尾素水平不仅统计学上增加，而且 TNF-α、IL-1β 和 IL-6 水平也降低。此外，鸢尾素蛋白的注射也可轻微减轻琼脂糖聚糖诱导的 SKG 小鼠的关节炎和附着点炎。同时，AS 患者的血清鸢尾素水平下降。总之，我们发现游泳运动可减轻 AS 动物模型中的病理性骨特征，可能是通过增加血清鸢尾素水平和相关的抗炎作用介导的。
