State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, School of Marine Sciences, Ningbo University, Ningbo, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, China.
J Fish Dis. 2024 Oct;47(10):e13996. doi: 10.1111/jfd.13996. Epub 2024 Jul 7.
Displaying antigens on yeast surface as an oral vaccine has been widely explored, while its potential as an immersion vaccine has not been evaluated. Here, an immersion vaccine was prepared by displaying ORF25 of Cyprinid herpesvirus 2 (CyHV-2) on the surface of Saccharomyces cerevisiae. Carassius auratus gibelio was immersion immunized by 2 × 10 CFU/mL yeast for 2 h, and reinforce the immunity using the same method 14 days after the first immunization. The results showed that ORF25 specific antibody in immunized crucian carp serum was detected at a high level, and the mRNA expression level of IgM, IgT, IL-1β, and IFN-1 in vaccinated head-kidney and spleen tissues were higher than the control group, indicating that innate and adaptive immunity were induced. Moreover, the immersion vaccination provided effective protection for fish against CyHV-2, leading to a relative percent survival of 50.2%. Meanwhile, immersion vaccination restrained virus replication and histological damage in CyHV-2 infected crucian carp. Our data suggested that immersion immunization of S. cerevisiae-displayed ORF25 could be served as a candidate vaccine to prevent CyHV-2 infection.
将抗原展示在酵母表面作为口服疫苗已经得到了广泛的研究，而其作为浸浴疫苗的潜力尚未得到评估。在这里，通过在酿酒酵母表面展示草鱼疱疹病毒 2（CyHV-2）的 ORF25，制备了一种浸浴疫苗。吉富罗非鱼经 2×10 CFU/mL 酵母浸浴 2 小时进行免疫，首次免疫 14 天后，采用相同方法进行加强免疫。结果表明，免疫鲫鱼血清中可检测到高水平的 ORF25 特异性抗体，接种鱼头肾和脾脏组织中 IgM、IgT、IL-1β和 IFN-1 的 mRNA 表达水平均高于对照组，表明诱导了先天和适应性免疫。此外，浸浴免疫为鱼类提供了针对 CyHV-2 的有效保护，导致相对存活率为 50.2%。同时，浸浴免疫抑制了 CyHV-2 感染鲫鱼中的病毒复制和组织学损伤。我们的数据表明，S. cerevisiae 展示的 ORF25 浸浴免疫可以作为预防 CyHV-2 感染的候选疫苗。
