Wen Yujie, Tang Cong, Shen Qing, Dong Shuqing, Wang Yaya, Li Yunchun, Shao Shijun
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China.
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, PR China.
Anal Methods. 2024 Jul 18;16(28):4865-4872. doi: 10.1039/d4ay00417e.
Developing an effective and convenient nitrite detection method is indispensable in food safety, environmental monitoring, clinical diagnosis of diseases, and many other areas. Herein, a dicyanoisophorone derivative, TMN-NH2 with large Stokes shift and near-infrared (NIR) emission, was proposed as a ratiometric fluorescence and colorimetric dual-mode probe for the rapid determination of NO in acidic media, showing excellent selectivity and high sensitivity. The sensing mechanism is based on the diazotization of TMN-NH2 with NO and subsequent diazonium salt hydrolysis to form a hydroxyl-substituted product (TMN-OH). Under the optimized conditions of reaction and detection, a new quantitative analysis method based on TMN-NH2 was established for NO detection, exhibiting good linear relationships to NO in the range of 0.5 to 15 μM with practical detection limits of 26.6 nM and 17.6 nM for the colorimetric and fluorescent readout, respectively. The quantitative detection of NO in real samples demonstrated satisfactory recoveries and repeatability. Moreover, TMN-NH2 was successfully applied for monitoring NO in by confocal fluorescence imaging.
开发一种有效且便捷的亚硝酸盐检测方法在食品安全、环境监测、疾病临床诊断及许多其他领域中不可或缺。在此，一种具有大斯托克斯位移和近红外（NIR）发射的二氰基异佛尔酮衍生物TMN-NH2被提出作为一种比率荧光和比色双模式探针，用于在酸性介质中快速测定NO，显示出优异的选择性和高灵敏度。传感机制基于TMN-NH2与NO的重氮化反应以及随后重氮盐水解形成羟基取代产物（TMN-OH）。在优化的反应和检测条件下，建立了一种基于TMN-NH2的NO检测新定量分析方法，该方法在0.5至15 μM范围内与NO呈现良好的线性关系，比色和荧光读数的实际检测限分别为26.6 nM和17.6 nM。实际样品中NO的定量检测显示出令人满意的回收率和重复性。此外，TMN-NH2通过共聚焦荧光成像成功应用于监测[具体内容缺失]中的NO。
