College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
Food Chem. 2022 Apr 16;374:131768. doi: 10.1016/j.foodchem.2021.131768. Epub 2021 Dec 3.
Nitrite (NO) is widely present in the human environment and accurate, sensitive and selective detecting of nitrite is of vital significance for food safety and water quality. Herein, a novel red emission carbon dots (r-CDs) fluorescent probe was fabricated for dual-mode detection of nitrite, which was capable of both convenient colorimetric analysis and accurate fluorometric detection. When NO is added to the rose-red r-CDs solution, NO interacts with the amino groups which on the surface of r-CDs to form diazotized substance, resulting in that the colorimetric color of r-CDs solution realizes the transition from rose red to light purple, and the red fluorescence is gradually quenched. The detection limits of colorimetric and fluorescence for NO were 0.193 μM and 0.149 μM, respectively. Furthermore, the dual-readout probe revealed satisfactory recovery and reliability when analyzing the concentration of NO in ham and bacon samples..
亚硝酸盐（NO）广泛存在于人类环境中，因此准确、灵敏、选择性地检测亚硝酸盐对于食品安全和水质至关重要。在此，我们构建了一种新型的红色发射碳点（r-CDs）荧光探针，用于亚硝酸盐的双模式检测，可实现比色分析和荧光检测的精确结合。当向玫瑰红色的 r-CDs 溶液中添加 NO 时，NO 与 r-CDs 表面的氨基相互作用形成重氮物质，导致 r-CDs 溶液的比色颜色从玫瑰红转变为浅紫色，红色荧光逐渐猝灭。比色和荧光检测 NO 的检测限分别为 0.193 μM 和 0.149 μM。此外，该双读数探针在分析火腿和培根样品中的 NO 浓度时表现出令人满意的回收率和可靠性。
