Analytical and Environmental Science Division & Centralized Instrument Facility, CSIR-Central Salt and Marine Chemicals Research Institute, Gijubhai Badheka Marg, Bhavnagar 364001, Gujarat, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad-201002, India.
Anal Methods. 2024 Jul 25;16(29):4971-4980. doi: 10.1039/d4ay00789a.
Detecting chronic autoimmune disorders (ADs) early reduces the risk of morbidity, disability, and mortality and offers the possibility of significant therapeutic action in a timely manner. Developing low-cost, reliable, and sensitive sensors for ADs can ensure the efficient utilization of healthcare resources at earlier stages. Here, we report on the development of an electrochemical biosensor for sensing CXCL10, a chemokine protein that serves as a biomarker for autoimmune diseases. A self-assembly strategy is used for the immobilization of biorecognition elements on a plastic chip electrode (PCE). A homemade PCE offers a versatile and cost-effective scaffold for sensing applications. Gold nanoparticles were electrochemically deposited on the electrode the reduction of gold ions on the PCE galvanostatically. The CXCL10 antibody and recognition elements were immobilized on the gold-deposited PCE. The attachment of recognition molecules was confirmed by energy-dispersive scanning electron microscopy, atomic force microscopy, infrared spectroscopy, and electrochemical techniques. Electrochemical impedance spectroscopy (EIS) was used for the detection of CXCL10 within a concentration range spanning from pico- to micro-molar levels. The sensor exhibited remarkable linearity in both buffer and plasma solutions, with a limit of detection (LOD) of up to 0.72 pg mL.
早期发现慢性自身免疫性疾病（AD）可以降低发病率、残疾率和死亡率的风险，并为及时采取重大治疗措施提供可能。开发用于 AD 的低成本、可靠且灵敏的传感器可以确保在早期阶段更有效地利用医疗保健资源。在这里，我们报告了一种用于检测趋化因子蛋白 CXCL10 的电化学生物传感器的开发，该蛋白作为自身免疫性疾病的生物标志物。采用自组装策略将生物识别元件固定在塑料芯片电极（PCE）上。自制的 PCE 为传感应用提供了一种通用且具有成本效益的支架。通过恒电流电化学在电极上沉积金纳米粒子，还原 PCE 上的金离子。将 CXCL10 抗体和识别元件固定在金沉积的 PCE 上。通过能量色散扫描电子显微镜、原子力显微镜、红外光谱和电化学技术确认了识别分子的附着。电化学阻抗谱（EIS）用于检测纳摩尔至微摩尔浓度范围内的 CXCL10。该传感器在缓冲液和血浆溶液中均表现出显著的线性，检测限（LOD）高达 0.72 pg mL。
