Kaji Takuya, Nishizato Yuho, Yoshimatsu Hidenori, Yoda Akiyoshi, Liang Wenting, Chini Andrea, Fernández-Barbero Gemma, Nozawa Kei, Kyozuka Junko, Solano Roberto, Ueda Minoru
Graduate School of Science, Tohoku University, 6-3, Aramaki-Aza-Aoba, Aoba-ku, Sendai 980-8578, Japan.
Graduate School of Life Sciences, Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai 980-8577, Japan.
iScience. 2024 Jun 6;27(7):110191. doi: 10.1016/j.isci.2024.110191. eCollection 2024 Jul 19.
Significant progress has been recently made in our understanding of the evolution of jasmonates biosynthesis and signaling. The bioactive jasmonate activating COI1-JAZ co-receptor differs in bryophytes and vascular plants. Dinor-12-oxo-phytodienoic acid (dn--OPDA) is the bioactive hormone in bryophytes and lycophytes. However, further studies showed that the full activation of hormone signaling in requires additional unidentified hormones. Δ-dn-OPDAs were previously identified as novel bioactive jasmonates in . In this paper, we describe the major bioactive isomer of Δ-dn-OPDAs as Δ-dn--OPDA through chemical synthesis, receptor binding assay, and biological activity in . In addition, we disclosed that Δ-dn--OPDA is a biosynthetic precursor of Δ-dn--OPDA. We demonstrated that in planta -to- conversion of Δ-dn--OPDA occurs in the biosynthesis of Δ-dn--OPDA, defining a key biosynthetic step in the chemical evolution of hormone structure. We predict that these findings will facilitate further understanding of the molecular evolution of plant hormone signaling.
最近，我们对茉莉酸生物合成和信号传导的进化有了重大进展。激活COI1-JAZ共受体的生物活性茉莉酸在苔藓植物和维管植物中有所不同。二氢-12-氧代-植物二烯酸（dn-OPDA）是苔藓植物和石松类植物中的生物活性激素。然而，进一步的研究表明，在[具体植物或系统]中激素信号的完全激活需要额外的未知激素。Δ-dn-OPDAs先前被鉴定为[具体植物或系统]中的新型生物活性茉莉酸。在本文中，我们通过化学合成、受体结合测定和[具体植物或系统]中的生物活性，将Δ-dn-OPDAs的主要生物活性异构体描述为Δ-dn-OPDA。此外，我们还揭示了Δ-dn-OPDA是Δ-dn-OPDA的生物合成前体。我们证明，在植物中，Δ-dn-OPDA向Δ-dn-OPDA的转化发生在Δ-dn-OPDA的生物合成过程中，这定义了激素结构化学进化中的一个关键生物合成步骤。我们预测，这些发现将有助于进一步理解植物激素信号传导的分子进化。
