家蚕 UFL1 通过调控宿主细胞凋亡促进 BmNPV 增殖。
Bombyx mori UFL1 facilitates BmNPV proliferation by regulating host cell apoptosis through PERK.
机构信息
School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Anhui Province Key Laboratory of Resource Insect Biology and Innovative Utilization, School of Life Sciences, Anhui Agricultural University, Hefei, China.
出版信息
Arch Insect Biochem Physiol. 2024 Jul;116(3):e22127. doi: 10.1002/arch.22127.
Ubiquitin-fold modifier 1 (UFM1) is attached to protein substrates through the sequential activity of an E1 (UBA5)-E2 (UFC1)-E3 (UFL1) cascade. UFL1 is the E3 ligase for UFMylation in vertebrates. However, there have been no studies on UFL1 in silkworm to date. In this study, we identified a UFL1 ortholog in Bombyx mori genome. Spatio-temporal expression profiles showed that BmUFL1 expression was high in the midgut, epidermis, and testis and in the pupa-adult stage. BmUFL1 knockdown inhibited B. mori nucleopolyhedrovirus (BmNPV) proliferation, while BmUFL1 overexpression promoted BmNPV proliferation. Mechanically, protein kinase RNA-like endoplasmic reticulum kinase (PERK) signaling and cell apoptosis are involved in BmUFL1-regulated BmNPV proliferation. Overall, these results suggest that BmUFL1 facilitates BmNPV proliferation in silkworm.
泛素样修饰物 1（UFM1）通过 E1（UBA5）-E2（UFC1）-E3（UFL1）级联反应将蛋白底物连接起来。UFL1 是脊椎动物 UFMylation 的 E3 连接酶。然而，迄今为止，在蚕中还没有关于 UFL1 的研究。在本研究中，我们在家蚕基因组中鉴定了一个 UFL1 同源物。时空表达谱显示，BmUFL1 在中肠、表皮和睾丸以及蛹-成虫期表达水平较高。BmUFL1 敲低抑制了家蚕核型多角体病毒（BmNPV）的增殖，而 BmUFL1 过表达促进了 BmNPV 的增殖。在机制上，蛋白激酶 RNA 样内质网激酶（PERK）信号和细胞凋亡参与了 BmUFL1 调控的 BmNPV 增殖。总的来说，这些结果表明 BmUFL1 在家蚕中促进了 BmNPV 的增殖。
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