División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, Mexico; CONAHCYT, Consejo Nacional de Humanidades, Ciencias y Tecnologías, Av. Insurgentes Sur 1582, Col. Crédito Constructor, Benito Juárez, Ciudad de México 03940, Mexico.
División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, Mexico.
J Pharm Biomed Anal. 2024 Oct 15;249:116334. doi: 10.1016/j.jpba.2024.116334. Epub 2024 Jul 3.
This work presents the modification of glassy carbon electrodes (GCE) by using a dispersion resulting from the non-covalent functionalization of multi-walled carbon nanotubes (MWCNT) with polyarginine (polyArg). MWCNT-polyArg is used for the quantification of ascorbic acid (AA) in the presence of acetaminophen (APAP) and viceversa. Since ascorbic acid and acetaminophen are strongly absorbed on GCE/MWCNT-polyArg, they can be detected in the presence of 4.0×10 M acetaminophen (and 3.0×10 M ascorbic acid) by using adsorptive stripping with media exchange and differential pulse voltammetry. Using water as the solvent for the MWCNT dispersion, the result was Z-potential of 0.053 ± 0.006 V. The developed sensor showed excellent specificity, sensitivity, stability and reproducibility compared to previously published sensors. The GCE/MWCNT-polyArg sensor shows a fast response time of ∼5 minutes, low limits of detection and quantification for AA (0.95 and 2.9 μM respectively) and APAP (0.27 and 0.82μM, respectively), high sensitivity of 0.0616 μA/M for AA or APAP 0.240μA/M. It was used to test its practicability by determining the concentration of AA or APAP (AA and APAP) in pharmaceutical samples. Finally, the simultaneous measurement of ascorbic acid and acetaminophen in pharmaceuticals showed a good correlation, with a maximum error and RSD of 4.5 and 5.1 %, respectively.
这项工作提出了通过使用多壁碳纳米管（MWCNT）与聚精氨酸（polyArg）的非共价功能化产生的分散体来修饰玻碳电极（GCE）。MWCNT-polyArg 用于在存在扑热息痛（APAP）的情况下定量测定抗坏血酸（AA），反之亦然。由于抗坏血酸和扑热息痛在 GCE/MWCNT-polyArg 上强烈吸附，因此可以通过使用带有介质交换和差分脉冲伏安法的吸附性剥离来在存在 4.0×10 M 扑热息痛（和 3.0×10 M 抗坏血酸）的情况下检测到它们。使用水作为 MWCNT 分散体的溶剂，得到的 Zeta 电位为 0.053±0.006 V。与以前发表的传感器相比，所开发的传感器具有出色的特异性、灵敏度、稳定性和重现性。GCE/MWCNT-polyArg 传感器具有快速的响应时间（约 5 分钟），AA（分别为 0.95 和 2.9 μM）和 APAP（分别为 0.27 和 0.82 μM）的低检测限和定量限，AA 或 APAP 的高灵敏度为 0.0616 μA/M 或 0.240μA/M。它通过测定药物样品中 AA 或 APAP（AA 和 APAP）的浓度来测试其实用性。最后，药物中抗坏血酸和扑热息痛的同时测量显示出良好的相关性，最大误差和 RSD 分别为 4.5%和 5.1%。
