Key Laboratory of Cold Water Fish Germplasm Resources and Multiplication and Cultivation of Heilongjiang Province, Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China.
Key Laboratory of Cold Water Fish Germplasm Resources and Multiplication and Cultivation of Heilongjiang Province, Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China; Center of Pharmaceutical Engineering and Technology, Harbin University of Commerce, Harbin 150076, PR China.
Int J Biol Macromol. 2024 Aug;275(Pt 2):133711. doi: 10.1016/j.ijbiomac.2024.133711. Epub 2024 Jul 6.
Ginger polysaccharides (GP) promote growth and development in fish. However, the effects of GP on crucian carp remain unclear. The present study investigated the effects of GP on the growth performance, immunity, intestinal microbiota, and disease resistance in crucian carp. Four treatment groups were established with different concentrations of GP (0.1 %, 0.2 %, 0.4 %, and 0.8 %). GP was not added as the control group, and the feeding period lasted for 56 d, followed by a 96-h anti-infection treatment using Aeromonas hydrophila. The results showed that dietary GP significantly improved growth performance, especially in the 0.4 % GP group. Furthermore, GP administration notably increased serum lysozyme (LMZ) activity, digestive enzyme performance, and antioxidant capacity of crucian carp. Moreover, dietary inclusion of GP up-regulated the expression of tumour necrosis factor-α (TNF-α), interleukin-8 (IL-8), interferon-γ (IFN-γ), and nuclear factor kappa-B (NF-κB) genes while down-regulating IL-10 and transforming growth factor-β (TGF-β) gene expressions, thus promoting liver health in crucian carp. Additionally, incorporating GP into the diet regulated both the diversity and composition of the intestinal microbiota in crucian carp, explicitly enhancing the relative abundance of beneficial bacteria, such as Fusobacteriota and Firmicutes. Therefore, GP reduces the mortality of crucian carp infected with A. hydrophila. In conclusion, this study provides novel insights into the application of dietary GP in cultured fish and evaluates the value of traditional Chinese medicinal polysaccharides against pathogenic bacteria.
姜多糖（GP）促进鱼类的生长和发育。然而，GP 对鲫鱼的影响尚不清楚。本研究探讨了 GP 对鲫鱼生长性能、免疫力、肠道微生物群和抗病能力的影响。建立了四个不同 GP 浓度（0.1%、0.2%、0.4%和 0.8%）的处理组。不添加 GP 作为对照组，饲养期为 56 天，然后用嗜水气单胞菌进行 96 小时的抗感染处理。结果表明，日粮 GP 显著提高了生长性能，特别是在 0.4% GP 组。此外，GP 给药显著提高了血清溶菌酶（LMZ）活性、消化酶性能和鲫鱼的抗氧化能力。此外，日粮添加 GP 上调了肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）、干扰素-γ（IFN-γ）和核因子 kappa-B（NF-κB）基因的表达，同时下调了白细胞介素-10 和转化生长因子-β（TGF-β）基因的表达，从而促进了鲫鱼的肝脏健康。此外，日粮中添加 GP 调节了鲫鱼肠道微生物群的多样性和组成，明确增强了有益细菌（如 Fusobacteriota 和 Firmicutes）的相对丰度。因此，GP 降低了感染嗜水气单胞菌的鲫鱼的死亡率。总之，本研究为在养殖鱼类中应用膳食 GP 提供了新的见解，并评估了传统中药多糖对抗致病菌的价值。
