Hong Heesang, Kim Hye-Jin, Kim Hyun-Jun, Jo Cheorun
Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 08826, Republic of Korea.
Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea.
Int Microbiol. 2025 Mar;28(3):447-460. doi: 10.1007/s10123-024-00556-0. Epub 2024 Jul 8.
This study explored the extracellular metabolomic responses of three different Salmonella enterica serotype Typhimurium (S. Typhimurium) strains-ATCC 13311 (STy1), NCCP 16964 (STy4), and NCCP 16958 (STy8)-cultured at refrigeration temperatures. The objective was to identify the survival mechanisms of S. Typhimurium under cold stress by analyzing variations in their metabolomic profiles. Qualitative and quantitative assessments identified significant metabolite alterations on day 6, marking a critical inflection point. Key metabolites such as trehalose, proline, glycerol, and tryptophan were notably upregulated in response to cold stress. Through multivariate analyses, the strains were distinguished using three metabolites-4-aminobutyrate, ethanol, and uridine-as potential biomarkers, underscoring distinct metabolic responses to refrigeration. Specifically, STy1 exhibited unique adaptive capabilities through enhanced metabolism of betaine and 4-aminobutyrate. These findings highlight the variability in adaptive strategies among S. Typhimurium strains, suggesting that certain strains may possess more robust metabolic pathways for enhancing survival in refrigerated conditions.
本研究探讨了三种不同的肠炎沙门氏菌鼠伤寒血清型菌株——ATCC 13311（STy1）、NCCP 16964（STy4）和NCCP 16958（STy8）在冷藏温度下培养时的细胞外代谢组学反应。目的是通过分析其代谢组学图谱的变化来确定鼠伤寒沙门氏菌在冷应激下的存活机制。定性和定量评估确定了第6天存在显著的代谢物变化，这标志着一个关键的转折点。诸如海藻糖、脯氨酸、甘油和色氨酸等关键代谢物在冷应激下显著上调。通过多变量分析，利用4-氨基丁酸、乙醇和尿苷这三种代谢物作为潜在生物标志物区分了这些菌株，突出了对冷藏的不同代谢反应。具体而言，STy1通过增强甜菜碱和4-氨基丁酸的代谢表现出独特的适应能力。这些发现突出了鼠伤寒沙门氏菌菌株之间适应策略的变异性，表明某些菌株可能拥有更强健的代谢途径以提高在冷藏条件下的存活率。
