测量细胞内钙离子浓度以研究 G 蛋白偶联受体介导的脂信号转导。
Measurement of Intracellular Ca for Studying GPCR-Mediated Lipid Signaling.
机构信息
Department of Graduate Nursing, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX, USA.
Bone and Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX, USA.
出版信息
Methods Mol Biol. 2024;2816:69-75. doi: 10.1007/978-1-0716-3902-3_7.
Intracellular Ca can be conveniently monitored by sensitive Ca fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca imaging. Here we describe the protocols to measure intracellular Ca for studying PEG2-EP1 activity in esophageal smooth muscle cells. The ratiometric Fura-2 imaging provides quantitative data, and the Fluo-4 confocal microscopic imaging has high-spatial resolution.
细胞内 Ca 可以通过活细胞中敏感的 Ca 荧光染料进行方便地监测。涉及脂质信号通路的 Gαq 可以通过细胞内 Ca 成像进行研究。本文描述了用于测量细胞内 Ca 以研究食管平滑肌细胞中 PEG2-EP1 活性的方案。比率型 Fura-2 成像提供定量数据，而 Fluo-4 共聚焦显微镜成像具有高空间分辨率。
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