胰岛素与培养的兔冠状动脉微血管内皮细胞的结合及胰岛素对葡萄糖摄取和代谢的影响。

Insulin binding and effects of insulin on glucose uptake and metabolism in cultured rabbit coronary microvessel endothelium.

作者信息

Gerritsen M E, Burke T M

出版信息

Proc Soc Exp Biol Med. 1985 Oct;180(1):17-23. doi: 10.3181/00379727-180-42137.

Abstract

Microvascular endothelial cells were isolated from rabbit cardiac tissue, and cultivated by standard tissue culture techniques. The conversion of [U-14C]glucose to CO2 and lipids was significantly enhanced by insulin treatment. Insulin stimulated uptake of 2-[3H]deoxyglucose and enhanced the transport of 3-O[3H]methyl-D-glucose. Specific binding of [125I]insulin to RCME cells, displaceable by cold insulin, was also observed. These data demonstrate that insulin is capable of regulating metabolic activities in coronary microvascular endothelium.

摘要

从兔心脏组织中分离出微血管内皮细胞，并采用标准组织培养技术进行培养。胰岛素处理显著增强了[U-14C]葡萄糖向二氧化碳和脂质的转化。胰岛素刺激了2-[3H]脱氧葡萄糖的摄取，并增强了3-O[3H]甲基-D-葡萄糖的转运。还观察到[125I]胰岛素与兔冠状动脉微血管内皮细胞（RCME细胞）的特异性结合，冷胰岛素可使其发生位移。这些数据表明胰岛素能够调节冠状动脉微血管内皮中的代谢活动。