植物全草提取物对 HPV18 感染的人宫颈癌（HeLa）细胞的分子作用。
Molecular Actions of Whole Plant Extract on HPV18-Infected Human Cervical Cancer (HeLa) Cells.
机构信息
Department of Biochemistry, Bharathiar University, Coimbatore, 641 046, India.
Department of Biotechnology, Maharani`s Science College for Women, 570005, Mysore, India.
出版信息
Anticancer Agents Med Chem. 2024;24(17):1253-1263. doi: 10.2174/0118715206296375240703115848.
OBJECTIVE
() contains several bioactive compounds with anti-cancer activities. This study was performed to investigate the molecular effects of on HPV18-containing HeLa cells.
METHODS
The methanol extract of whole plant was tested for cytotoxicity by MTT assay. A lower and higher dose (80 and 160 μg/mL) to IC were analyzed for colonization inhibition (Clonogenic assay), cell cycle arrest (FACS analysis), and induction of apoptosis (AO/EtBr staining fluorescent microscopy and FACS analysis) and DNA fragmentation (comet assay). The HPV 18 E6 gene expression in treated cells was analyzed using RT-PCR and qPCR.
RESULTS
A significant dose-dependent anti-proliferative activity (IC - 108.25±2 μg/mL) and inhibition of colony formation cell line were observed using both treatments. Treatment with 80 μg/mL of extract was found to result in a higher percent of cell cycle arrest at G/G and GM phases with more early apoptosis, while 160 μg/mL resulted in more cell cycle arrest at SUBG0 and S phases with late apoptosis for control. The comet assay also demonstrated a highly significant increase in DNA fragmentation after treatment with 160 μg/mL of extract (tail moments-19.536 ± 17.8), while 80 μg/mL of extract treatment showed non-significant tail moment (8.152 ± 13.0) compared to control (8.038 ± 12.0). The RT-PCR and qPCR results showed a significant reduction in the expression of the HPV18 E6 gene in HeLa cells treated with 160 μg/mL of extract, while 80 μg/mL did not show a significant reduction.
CONCLUSION
The 160 μg/mL methanol extract of demonstrated highly significant anti-cancer molecular effects in HeLa cells.
目的
（）含有几种具有抗癌活性的生物活性化合物。本研究旨在探讨（）对含 HPV18 的 HeLa 细胞的分子作用。
方法
采用 MTT 法检测（）全草甲醇提取物的细胞毒性。分析低剂量（80μg/ml）和高剂量（160μg/ml）对集落抑制（集落形成试验）、细胞周期阻滞（FACS 分析）、诱导凋亡（AO/EtBr 染色荧光显微镜和 FACS 分析）和 DNA 片段化（彗星试验）的影响。采用 RT-PCR 和 qPCR 分析处理细胞中 HPV18 E6 基因的表达。
结果
两种处理均观察到明显的剂量依赖性抗增殖活性（IC50-108.25±2μg/ml）和对细胞系集落形成的抑制作用。用 80μg/ml 的提取物处理后，G/G 和 GM 期的细胞周期阻滞率更高，早期凋亡率更高，而用 160μg/ml 的提取物处理后，SUBG0 和 S 期的细胞周期阻滞率更高，晚期凋亡率更高。彗星试验还表明，用 160μg/ml 提取物处理后，DNA 片段化显著增加（尾矩-19.536±17.8），而用 80μg/ml 提取物处理后，尾矩无显著变化（8.152±13.0），与对照组（8.038±12.0）相比。RT-PCR 和 qPCR 结果显示，用 160μg/ml 提取物处理后，HeLa 细胞中 HPV18 E6 基因的表达显著降低，而 80μg/ml 提取物处理后，HPV18 E6 基因的表达无显著降低。
结论
（）的 160μg/ml 甲醇提取物在 HeLa 细胞中表现出显著的抗癌分子作用。
Zotero 插件