von Loeffelholz Christian, Winkler René, Weigel Cynthia, Piskor Eva-Maria, Vivas Wolfgang, Rauchfuß Falk, Settmacher Utz, Rubio Ignacio, Weis Sebastian, Gräler Markus H, Bauer Michael, Kosan Christian
Department of Anesthesiology and Intensive Care, Jena University Hospital, Friedrich Schiller University, Am Klinikum 1, 07749 Jena, Germany.
Department of Biochemistry, Center for Molecular Biomedicine (CMB), Friedrich Schiller University, Hans-Knöll-Str. 2, 07745 Jena, Germany.
iScience. 2024 Jun 6;27(7):110133. doi: 10.1016/j.isci.2024.110133. eCollection 2024 Jul 19.
Sepsis is a life-threatening condition caused by dysregulated host responses to infection. Myeloid cell accumulation and lymphocyte decline are widely recognized phenomena in septic patients. However, the fate of specific immune cells remains unclear. Here, we report the results of a human explorative study of patients with septic peritonitis and patients undergoing abdominal surgery without sepsis. We analyzed pairwise peritoneal fluid and peripheral blood taken 24 h after surgery to characterize immediate immune cell changes. Our results show that myeloid cell expansion and lymphocyte loss occur in all patients undergoing open abdominal surgery, indicating that these changes are not specific to sepsis. However, B1-like lymphocytes were specifically increased in the peritoneal fluid of septic patients, correlating positively with sequential organ failure assessment (SOFA) and acute physiology and chronic health evaluation II (APACHE-II) clinical severity scores. In support of this notion, we identified an accumulation of peritoneal B1b lymphocytes in septic mice.
脓毒症是一种由宿主对感染的反应失调引起的危及生命的病症。髓样细胞积聚和淋巴细胞减少是脓毒症患者中广泛认可的现象。然而，特定免疫细胞的命运仍不清楚。在此，我们报告了一项对脓毒症性腹膜炎患者和未患脓毒症的腹部手术患者进行的人体探索性研究的结果。我们分析了术后24小时采集的成对腹膜液和外周血，以表征即时免疫细胞变化。我们的结果表明，所有接受开放性腹部手术的患者均出现髓样细胞扩增和淋巴细胞丢失，这表明这些变化并非脓毒症所特有。然而，脓毒症患者的腹膜液中B1样淋巴细胞特异性增加，与序贯器官衰竭评估（SOFA）和急性生理学与慢性健康状况评估II（APACHE-II）临床严重程度评分呈正相关。为支持这一观点，我们在脓毒症小鼠中发现了腹膜B1b淋巴细胞的积聚。
