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肽的单磷酸腺苷核糖基化：合成及化学酶法概述

Mono-ADP-Ribosylation of Peptides: An Overview of Synthetic and Chemoenzymatic Methodologies.

作者信息

Minnee Hugo, Codée Jeroen D C, Filippov Dmitri V

机构信息

Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, 2300 RA, Netherlands.

出版信息

Chembiochem. 2024 Dec 16;25(24):e202400440. doi: 10.1002/cbic.202400440. Epub 2024 Sep 5.

DOI:10.1002/cbic.202400440
PMID:38984757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11664928/
Abstract

Adenosine diphosphate (ADP)-ribosylation is a ubiquitous post-translational modification that regulates vital biological processes like histone reorganization and DNA-damage repair through the modification of various amino acid residues. Due to advances in mass-spectrometry, the collection of long-known ADP-ribose (ADPr) acceptor sites, e. g. arginine, cysteine and glutamic acid, has been expanded with serine, tyrosine and histidine, among others. Well-defined ADPr-peptides are valuable tools for investigating the exact structures, mechanisms of action and interaction partners of the different flavors of this modification. This review provides a comprehensive overview of synthetic and chemoenzymatic methodologies that enabled the construction of peptides mono-ADP-ribosylated on various amino acids, and close mimetics thereof.

摘要

二磷酸腺苷（ADP）-核糖基化是一种普遍存在的翻译后修饰，通过修饰各种氨基酸残基来调节诸如组蛋白重组和DNA损伤修复等重要生物学过程。由于质谱技术的进步，长期以来已知的ADP-核糖（ADPr）受体位点，如精氨酸、半胱氨酸和谷氨酸，已扩展到丝氨酸、酪氨酸和组氨酸等。明确的ADPr肽是研究这种修饰不同形式的确切结构、作用机制和相互作用伙伴的宝贵工具。本综述全面概述了合成和化学酶促方法，这些方法能够构建在各种氨基酸上单ADP-核糖基化的肽及其紧密模拟物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6963/11664928/dccc339ecb2f/CBIC-25-e202400440-g013.jpg
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