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提高蓝莓冷藏质量:采前己醛处理对冷害及抗氧化防御机制的影响。

Improving blueberry cold storage quality: the effect of preharvest hexanal application on chilling injuries and antioxidant defense mechanisms.

机构信息

Yaltır Agricultural Products Sarıhuğlar, Adana, Turkey.

Department of Food Engineering, Engineering Faculty, Osmaniye Korkut Ata University, Osmaniye, Turkey.

出版信息

J Sci Food Agric. 2024 Nov;104(14):8837-8851. doi: 10.1002/jsfa.13710. Epub 2024 Jul 10.

DOI:10.1002/jsfa.13710
PMID:38985128
Abstract

Blueberries are vulnerable to chilling injury (CI). This can lead to limited longevity when they are subjected to cold storage conditions. This study investigated the effectiveness of a preharvest spray containing 0.02% hexanal in reducing CI and improving the postharvest storage quality of 'Star' and 'Biloxi' blueberries. The blueberries were stored for a period of 5 weeks at 2 °C and in 90% relative humidity (RH). The findings revealed that the preharvest hexanal spraying of both cultivars delayed senescence by mitigating CI, as evidenced by the bolstering of the antioxidant defense system through increased superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase (POD), catalase (CAT), and phenylalanine ammonia lyase (PAL) enzyme activity. The treated fruit also maintained elevated levels of total phenol content (TPC), total flavonoids (TFC), and vitamin C, demonstrating enhanced free radical scavenging capacity (FRSC), while exhibiting reduced polyphenoloxidase (PPO) activity, and reduced malondialdehyde (MDA), and HO content in comparison with the control group. The preharvest hexanal treatment also suppressed fruit softening by maintaining greater firmness and higher membrane stability index (MSI) scores, inhibiting the activity of polygalacturonase (PG), pectinmethylesterase (PME), xylanase, and α-amylase, and reducing microbial counts (MC) and incidence of decay (DI) in comparison with the control. Preharvest hexanal treatment also improved the overall storage quality by reducing weight loss, total soluble solids (TSS), pH, and the TSS/acid ratio, while increasing titratable acidity (TA) in comparison with the control during cold storage. The findings suggest that hexanal, as a preharvest application, delays senescence effectively and preserves overall quality by enhancing cold tolerance through antioxidant defense mechanisms in blueberry storage under cold conditions. © 2024 The Author(s). Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

摘要

蓝莓易受冷害(CI)影响。在冷藏条件下,蓝莓的贮藏寿命会因此受到限制。本研究探讨了采前喷施 0.02%己醛对减少 CI 和提高‘Star’和‘Biloxi’蓝莓采后贮藏品质的效果。将蓝莓在 2°C 和 90%相对湿度(RH)下贮藏 5 周。结果表明,两种品种的采前己醛喷雾均通过缓解 CI 来延缓衰老,这表现在通过增加超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)、过氧化氢酶(CAT)和苯丙氨酸解氨酶(PAL)酶活性来增强抗氧化防御系统。处理过的果实还保持较高的总酚含量(TPC)、总类黄酮(TFC)和维生素 C,表现出增强的自由基清除能力(FRSC),同时多酚氧化酶(PPO)活性降低,丙二醛(MDA)和 HO 含量降低,与对照组相比。采前己醛处理还通过保持较高的硬度和较高的膜稳定性指数(MSI)评分来抑制多聚半乳糖醛酸酶(PG)、果胶甲酯酶(PME)、木聚糖酶和α-淀粉酶的活性,降低微生物计数(MC)和腐烂发生率(DI),从而抑制果实软化,与对照组相比。采前己醛处理还通过降低冷藏过程中的失重、总可溶性固形物(TSS)、pH 值和 TSS/酸比,同时增加可滴定酸度(TA),来提高整体贮藏品质,与对照组相比。结果表明,己醛作为一种采前处理剂,通过抗氧化防御机制增强蓝莓对冷胁迫的耐受性,有效地延缓衰老,保持整体品质。© 2024 作者。约翰威立父子公司代表化学工业学会出版的《食品科学杂志》刊登的文章。

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