Grupo Especializado de Apoyo a Biofábrica, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.
CEIBASS-Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.
Methods Mol Biol. 2024;2827:155-163. doi: 10.1007/978-1-0716-3954-2_10.
This chapter presents the methodological approach for the in vitro propagation of Agave angustifolia "espadin," the base material to produce mezcal. The protocol used in each stage of the crop is addressed in detail, considering the changes in the culture medium and the characteristics of the plant material at each stage. The importance of integrated management between the multiplication and growth phase, as part of the in vitro selection strategy, is mentioned.
本章介绍了龙舌兰“espadin”离体繁殖的方法,龙舌兰是生产梅斯卡尔酒的基础材料。详细介绍了作物各个阶段使用的方案,考虑了培养基的变化和每个阶段植物材料的特点。还提到了在离体选择策略的一部分中,繁殖和生长阶段之间的综合管理的重要性。