Gonçalves Letícia Almeida, Geraldine Robson Maia, Felipe Sérgio Heitor Sousa, Sousa Elisandra Silva, Henschel Juliane Maciel, Batista Diego Silva, Azevedo Aristéa Alves, Otoni Wagner Campos
Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil.
Escola de Agronomia, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil.
Methods Mol Biol. 2024;2827:179-187. doi: 10.1007/978-1-0716-3954-2_12.
An efficient procedure for in vitro propagation of Herreria salsaparrilha Martius was established from single-node explants (fourth and fifth nodes from apex to the base) derived from donor plants maintained under shading-house conditions. After surface sterilization, explants are inoculated in test tubes containing 15 mL of Murashige and Skoog (MS) medium without growth regulators. Cultures are maintained under 35 μmol m s irradiance, a 16/8-h light/dark light regime, at 26 ± 2 °C. The subcultures are carried out under the same conditions, adding 6-benzyladenine 1.0 mg/L and Phytagel 2.8 g/L. Shoots are elongated and rooted by transferring individual shoots to half-strength MS medium without growth regulators. After 25-30 days, elongated rooted shoots are transferred to plastic pots containing 25-30 mL of sterile distilled water, covered with a transparent plastic bag, and kept under the same growth room conditions for 2 days. Plants are transferred to cups containing autoclaved and washed sand and kept in a shading house (50% light interception) for acclimatization. True-to-type adult plants were successfully recovered under ex vitro conditions.
建立了一种从遮光条件下生长的供体植物的单节外植体（从顶端到基部的第四和第五个节）中高效繁殖 Herreria salsaparrilha Martius 的方法。经过表面消毒后，将外植体接种于不含生长调节剂的 15 mL Murashige 和 Skoog (MS) 培养基的试管中。在 35 μmol m s 光照强度、16/8 小时光照/黑暗光周期和 26 ± 2°C 的条件下培养。在相同条件下进行继代培养，添加 1.0 mg/L 6-苄基腺嘌呤和 2.8 g/L Phytagel。通过将单个芽转移到不含生长调节剂的半强度 MS 培养基中，使芽伸长并生根。25-30 天后，将伸长的生根芽转移到含有 25-30 mL 无菌蒸馏水的塑料盆中，用透明塑料袋覆盖，并在相同的生长室条件下保持 2 天。将植物转移到含有高压灭菌和洗涤砂的杯子中，并在遮荫室（50%的光截留）中进行驯化。在室外条件下成功恢复了典型的成年植物。
