Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Department of Biological Sciences, Columbia University, New York, New York 10027, USA
RNA. 2024 Aug 16;30(9):1122-1140. doi: 10.1261/rna.080108.124.
The cleavage and polyadenylation specificity factor (CPSF) complex plays a central role in the formation of mRNA 3' ends, being responsible for the recognition of the poly(A) signal sequence, the endonucleolytic cleavage step, and recruitment of poly(A) polymerase. CPSF has been extensively studied for over three decades, and its functions and those of its individual subunits are becoming increasingly well-defined, with much current research focusing on the impact of these proteins on the normal functioning or disease/stress states of cells. In this review, we provide an overview of the general functions of CPSF and its subunits, followed by a discussion of how they exert their functions in a surprisingly diverse variety of biological processes and cellular conditions. These include transcription termination, small RNA processing, and R-loop prevention/resolution, as well as more generally cancer, differentiation/development, and infection/immunity.
剪接多聚腺苷酸化特异性因子（CPSF）复合物在 mRNA 3' 末端的形成中起着核心作用，负责识别多 A 信号序列、内切核酸酶切割步骤以及募集多 A 聚合酶。CPSF 已经被广泛研究了三十多年，其功能及其各个亚基的功能越来越明确，目前的许多研究都集中在这些蛋白质对细胞正常功能或疾病/应激状态的影响上。在这篇综述中，我们概述了 CPSF 及其亚基的一般功能，然后讨论了它们在各种不同的生物学过程和细胞条件下如何发挥作用。这些过程包括转录终止、小 RNA 加工和 R 环的预防/解决，以及更普遍的癌症、分化/发育和感染/免疫。
