Impact of biomass on the viability of subsp. strain during the freeze-drying process.

In this work, we evaluated the protective capacity of biomass in preserving subsp. WDCM 00102. The strain was freeze-dried in the presence of biomass and the freeze-dried samples were then stored at 5 and 25°C for varying periods. Subsequently, the samples were rehydrated and bacterial plate counts were determined. The results indicate that a concentration of 12% biomass was highly effective in preserving . Commercial products with higher biomass content exhibited greater protective capacity. While biomass is well-known for its prebiotic properties, its protective role has not been previously reported or thoroughly explored. This study demonstrates the protective capacity of biomass in preserving , a strain particularly sensitive to preservation processes.