Hazato T, Shimamura M, Kase R, Iijima M, Katayama T
Biochem Pharmacol. 1985 Sep 1;34(17):3179-83. doi: 10.1016/0006-2952(85)90166-2.
The enkephalin-degrading enzymes, such as aminopeptidase, dipeptidyl aminopeptidase, dipeptidyl carboxypeptidase and carboxypeptidase, were purified partially by DEAE-cellulose column chromatography, using longitudinal muscle from bovine small intestine. These enzyme were inhibited by EDTA and o-phenanthroline. Several protease inhibitors of microbial origin, and synthetic compounds, were tested for their abilities to inhibit these enkephalin-degrading enzymes. Among them, arphamenine A, a new potent inhibitor for aminopeptidase B, was shown to be a useful compound in inhibiting all of the enkephalin-degrading enzymes in small intestine.
使用牛小肠纵肌，通过DEAE - 纤维素柱色谱法对脑啡肽降解酶，如氨肽酶、二肽基氨肽酶、二肽基羧肽酶和羧肽酶进行了部分纯化。这些酶受到EDTA和邻菲罗啉的抑制。测试了几种微生物来源的蛋白酶抑制剂和合成化合物抑制这些脑啡肽降解酶的能力。其中，阿法美宁A是一种新型强效氨肽酶B抑制剂，被证明是一种有效抑制小肠中所有脑啡肽降解酶的化合物。
