Charpin C, Lissitzky J C, Kopp F, Jacquemier J, Lavaut M N, Pourreau-Schneider N, Martin P M, Toga M
Ann Pathol. 1985;5(2):77-84.
A wide range of normal human tissue samples including cervix, endometrium, thyroid, pancreas, parotid, breast, placenta, gastric mucosae, striated muscle were compared with tumorous and non tumorous disorders (thyroiditis, Graves disease, follicular adenoma, thyroid carcinomas, breast cystic disease, fibroadenoma, adenosis, breast carcinomas) using anti-laminin and Avidin Biotin Peroxidase complex method on frozen sections (light microscopy study) and vibratome cut 100 micrometer-thick-sections (electron microscopy study). It was shown that laminin was located in the lamina densa of basement membranes (BM) in normal human tissue and visible on BM like structures around decidua cells, BM were abnormally thick and often multilayered but continuous and laminin positive in intraductal breast carcinomas and well differentiated follicular carcinomas of thyroid, in invasive carcinomas laminin immunostaining displayed an heterogeneous pattern with disruptions and even may completely disappeared, in tumor stroma, blood vessels BM had a laminin abnormal staining with a multilayered pattern. Since laminin is involved in cell attachment to basement membrane through specific receptors to laminin and to biochemical components of modified interstitium found in tumorous disorders, laminin immunohistochemical detection constitutes a valuable method for a better understanding of tumor cells diffusion and metastases development.
使用抗层粘连蛋白和抗生物素蛋白-生物素过氧化物酶复合物方法，在冰冻切片（光学显微镜研究）和振动切片机切取的100微米厚切片（电子显微镜研究）上，将包括子宫颈、子宫内膜、甲状腺、胰腺、腮腺、乳腺、胎盘、胃黏膜、横纹肌在内的多种正常人体组织样本与肿瘤性和非肿瘤性疾病（甲状腺炎、格雷夫斯病、滤泡性腺瘤、甲状腺癌、乳腺囊性疾病、纤维腺瘤、腺病、乳腺癌）进行比较。结果表明，层粘连蛋白位于正常人体组织基底膜的致密层，在蜕膜细胞周围的基底膜样结构上可见，在乳腺导管内癌和甲状腺高分化滤泡癌中，基底膜异常增厚且常为多层，但连续且层粘连蛋白呈阳性，在浸润性癌中，层粘连蛋白免疫染色呈现异质性模式，有中断甚至可能完全消失，在肿瘤间质中，血管基底膜层粘连蛋白染色异常，呈多层模式。由于层粘连蛋白通过层粘连蛋白特异性受体以及在肿瘤性疾病中发现的修饰间质的生化成分参与细胞与基底膜的附着，层粘连蛋白免疫组织化学检测是一种有价值的方法，有助于更好地理解肿瘤细胞的扩散和转移发展。
