A method for anterograde axonal tracing of chemically specified circuits in the central nervous system: combined Phaseolus vulgaris-leucoagglutinin (PHA-L) tract tracing and immunohistochemistry.

A method is described for combining anterograde axonal tract tracing using concurrent double immunohistochemical localization of axonally transported Phaseolus vulgaris-leucoagglutinin (PHA-L) and endogenous neural antigens. With this technique, some projections of the substantia nigra and the paraventricular hypothalamic nucleus are examined to demonstrate advantages offered by this approach for tracing chemically specified circuits in the central nervous system.