一种用于人类外周血单核细胞的 38 色高维免疫表型分析面板。
A 38-colour high dimensional immunophenotyping panel for human peripheral blood mononuclear cells.
机构信息
Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, University of Melbourne, Melbourne, Australia.
Murdoch Children's Research Institute, Melbourne, Australia.
出版信息
J Immunol Methods. 2024 Sep;532:113726. doi: 10.1016/j.jim.2024.113726. Epub 2024 Jul 9.
High dimensional immunophenotyping panels are invaluable resources for performing extensive phenotyping on peripheral blood mononuclear cells (PBMCs). We designed a 38-colour high dimensional phenotyping panel to measure innate (monocytes, dendritic cells, NK cells, basophils, innate like lymphoid cells), T cell (γδ T cells, MAIT cells, CD4 and CD8 memory, Th1, Th2, Th17, Tfh, Treg) and B cell (memory, plasma cells, transitional B cells, plasmablasts, IgG, IgM) subsets in addition to their activation status using the 5-laser Cytek Aurora. We optimised optimal fluorochrome combinations and titres to minimise spread and autofluorescence of rare immune cell populations and tested this panel on PBMCs from 15 healthy adults. This high dimensional panel will be invaluable for direct ex vivo studies to evaluate immune cells in the context of human health and disease, especially when samples are limited.
高维免疫表型面板是对外周血单个核细胞（PBMC）进行广泛表型分析的宝贵资源。我们设计了一个 38 色的高维免疫表型面板，使用 5 激光 Cytek Aurora 来测量先天（单核细胞、树突状细胞、NK 细胞、嗜碱性粒细胞、先天样淋巴细胞）、T 细胞（γδ T 细胞、MAIT 细胞、CD4 和 CD8 记忆细胞、Th1、Th2、Th17、Tfh、Treg）和 B 细胞（记忆细胞、浆细胞、过渡 B 细胞、浆母细胞、IgG、IgM）亚群及其激活状态。我们优化了最佳的荧光染料组合和滴度，以最小化稀有免疫细胞群体的扩散和自发荧光，并在 15 名健康成年人的 PBMC 上测试了这个面板。这个高维面板对于直接在体外研究评估人类健康和疾病背景下的免疫细胞非常有价值，尤其是在样本有限的情况下。
Zotero 插件