Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109.
Cytometry A. 2018 Apr;93(4):402-405. doi: 10.1002/cyto.a.23331. Epub 2018 Jan 22.
This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size.
本研究采用基于荧光的流式细胞术对人类树突状细胞（DC）亚群进行了首次 30 参数免疫表型分析。优化后的面板可同时检测区分所有经典 DC 亚群的 21 个髓系细胞标志物，同时平行计数单核细胞、T 细胞和 B 细胞以及 NK 细胞。因此，该面板将有助于对各种大小有限的人类样本中的免疫细胞进行广泛的表型分析。
