State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
National Center for Stomatology, Sichuan University, Chengdu 610041, China.
ACS Biomater Sci Eng. 2024 Aug 12;10(8):5108-5121. doi: 10.1021/acsbiomaterials.4c00569. Epub 2024 Jul 12.
Commercial mammalian collagen-based membranes used for guided tissue regeneration (GTR) in periodontal defect repair still face significant challenges, including ethical concerns, cost-effectiveness, and limited capacity for periodontal bone regeneration. Herein, an enhanced biomimetic mineralized hydroxyapatite (HAp)-fish-scale collagen (FCOL)/chitosan (CS) nanofibrous membrane was developed. Specifically, eco-friendly and biocompatible collagen extracted from grass carp fish scales was co-electrospun with CS to produce a biomimetic extracellular matrix membrane. An enhanced biomimetic mineralized HAp coating provided abundant active calcium and phosphate sites, which promoted cell osteogenic differentiation, and showed greater in vivo absorption. In vitro experiments demonstrated that the HAp-FCOL/CS membranes exhibited desirable properties with no cytotoxicity, provided a mimetic microenvironment for stem cell recruitment, and induced periodontal ligament cell osteogenic differentiation. In rat periodontal defects, HAp-FCOL/CS membranes significantly promoted new periodontal bone formation and regeneration. The results of this study indicate that low-cost, eco-friendly, and biomimetic HAp-FCOL/CS membranes could be promising alternatives to GTR membranes for periodontal regeneration in the clinic.
商业化的哺乳动物胶原基膜在牙周缺损修复中的引导组织再生（GTR）方面仍面临重大挑战，包括伦理问题、成本效益和牙周骨再生能力有限。本文开发了一种增强型仿生矿化羟基磷灰石（HAp）-鱼鳞胶原蛋白（FCOL）/壳聚糖（CS）纳米纤维膜。具体来说，从草鱼鱼鳞中提取的环保且生物相容的胶原蛋白与 CS 共电纺，以产生仿生细胞外基质膜。增强型仿生矿化 HAp 涂层提供了丰富的活性钙和磷酸盐位点，促进了细胞成骨分化，并显示出更大的体内吸收。体外实验表明，HAp-FCOL/CS 膜具有良好的性能，无细胞毒性，为干细胞募集提供了仿生微环境，并诱导牙周韧带细胞成骨分化。在大鼠牙周缺损中，HAp-FCOL/CS 膜显著促进了新的牙周骨形成和再生。本研究结果表明，低成本、环保且仿生的 HAp-FCOL/CS 膜可能是 GTR 膜在临床牙周再生中的有前途的替代品。
