School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, China.
Shenzhen Baoan Authentic TCM Therapy Hospital, Shenzhen, Guangdong, 518101, China.
Talanta. 2024 Oct 1;278:126496. doi: 10.1016/j.talanta.2024.126496. Epub 2024 Jul 11.
Dopamine is an important neurotransmitter in the body and closely related to many neurodegenerative diseases. Therefore, the detection of dopamine is of great significance for the diagnosis and treatment of diseases, screening of drugs and unraveling of relevant pathogenic mechanisms. However, the low concentration of dopamine in the body and the complexity of the matrix make the accurate detection of dopamine challenging. Herein, an electrochemical sensor is constructed based on ternary nanocomposites consisting of one-dimensional Pt nanowires, two-dimensional MXene nanosheets, and three-dimensional porous carbon. The Pt nanowires exhibit excellent catalytic activity due to the abundant grain boundaries and highly undercoordinated atoms; MXene nanosheets not only facilitate the growth of Pt nanowires, but also enhance the electrical conductivity and hydrophilicity; and the porous carbon helps induce significant adsorption of dopamine on the electrode surface. In electrochemical tests, the ternary nanocomposite-based sensor achieves an ultra-sensitive detection of dopamine (S/N = 3) with a low limit of detection (LOD) of 28 nM, satisfactory selectivity and excellent stability. Furthermore, the sensor can be used for the detection of dopamine in serum and in situ monitoring of dopamine release from PC12 cells. Such a highly sensitive nanocomposite sensor can be exploited for in situ monitoring of important neurotransmitters at the cellular level, which is of great significance for related drug screening and mechanistic studies.
多巴胺是体内一种重要的神经递质，与许多神经退行性疾病密切相关。因此，多巴胺的检测对于疾病的诊断和治疗、药物筛选以及相关致病机制的揭示都具有重要意义。然而，由于体内多巴胺浓度低且基质复杂，使得多巴胺的准确检测具有挑战性。在此，构建了一种基于由一维 Pt 纳米线、二维 MXene 纳米片和三维多孔碳组成的三元纳米复合材料的电化学传感器。Pt 纳米线由于丰富的晶界和高度配位不足的原子而表现出优异的催化活性；MXene 纳米片不仅有利于 Pt 纳米线的生长，而且增强了电导率和亲水性；多孔碳有助于诱导多巴胺在电极表面的显著吸附。在电化学测试中，基于三元纳米复合材料的传感器对多巴胺实现了超灵敏检测（S/N=3），检测限（LOD）低至 28 nM，具有令人满意的选择性和出色的稳定性。此外，该传感器可用于血清中多巴胺的检测以及 PC12 细胞中多巴胺释放的原位监测。这种高灵敏度的纳米复合传感器可用于在细胞水平上对重要神经递质进行原位监测，这对于相关药物筛选和机制研究具有重要意义。
