在器官培养中研究钙和胰岛素对小鼠乳腺DNA合成的调节作用。

Calcium and insulin in the regulation of DNA synthesis in mouse mammary gland studied in organ culture.

作者信息

Zwierzchowski L, Rynca J, Niedbalski W

出版信息

Mol Cell Endocrinol. 1985 Oct;42(3):221-5. doi: 10.1016/0303-7207(85)90052-8.

Abstract

The interaction of calcium and insulin was investigated in mouse mammary gland organ culture by analysis of the time-course of the DNA-synthetic response following delayed addition of insulin or calcium to the culture media. The DNA-synthetic responses were also studied using mammary explants from virgin, pregnant and lactating mice. These experiments have shown that qualitative differences exist in the responsiveness of mammary epithelium to insulin and calcium. Thus, although it is evident that in mammary epithelium calcium modifies the DNA-synthetic response to insulin, the present data do not support the concept that Ca2+ is the intracellular mediator of insulin action.

摘要

通过分析在培养基中延迟添加胰岛素或钙后DNA合成反应的时间进程，在小鼠乳腺器官培养中研究了钙与胰岛素的相互作用。还使用来自处女、怀孕和泌乳小鼠的乳腺外植体研究了DNA合成反应。这些实验表明，乳腺上皮对胰岛素和钙的反应性存在质的差异。因此，虽然很明显在乳腺上皮中钙会改变对胰岛素的DNA合成反应，但目前的数据并不支持Ca2+是胰岛素作用的细胞内介质这一概念。