Adi Yosua Kristian, Taechamaeteekul Preechaphon, Kesdangsakonwut Sawang, Tienthai Paisan, Kirkwood Roy N, Tummaruk Padet
Centre of Excellence in Swine Reproduction, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Animals (Basel). 2024 Jun 21;14(13):1846. doi: 10.3390/ani14131846.
This study investigated the gross morphology of reproductive organs and oxytocin receptor distribution across different parities in hyperprolific sows. A total of thirty-two reproductive organs from Landrace × Yorkshire crossbred sows were categorized into three groups based on parity numbers: 1 ( = 10), 2-5 ( = 12), and ≥6 ( = 10). All sows were culled due to management problems, and none had reproductive disorders. A gross morphology examination of the ovaries, uterus, and the rest of the reproductive tract was conducted. Using immunohistochemistry, the levels of oxytocin receptor were evaluated in five layers of the uterus, the epithelial, superficial glandular, deep glandular, and circular and longitudinal smooth muscles of the myometrium, and were quantified using an H-score. On average, the age and body weight of sows and the total number of piglets born per litter were 799.8 ± 327.8 days, 213.2 ± 31.7 kg, and 15.5 ± 4.8, respectively. The numbers of ovulations in sows in parity number 1 (19.9 ± 2.4) were lower than those in sows in parity numbers 2-5 (29.7 ± 2.0, = 0.004) and ≥6 (27.7 ± 2.1, = 0.022). The uterine weights of sows in parity number 1 (902.9 ± 112.5 g) were lower than those of parity numbers 2-5 (1442.1 ± 111.8 g, = 0.001) and ≥6 (1394.3 ± 125.1 g, = 0.004). The length of the uterus in sows with parity number 1 (277.9 ± 26.1 cm) was shorter than those in sows with parity numbers 2-5 (354.6 ± 25.9 cm, = 0.033) and tended to be shorter than those in sows with parity numbers ≥ 6 (346.6 ± 29.0 cm, = 0.068). The immunolocalization of oxytocin receptors could be detected in various parts of the porcine endometrium and myometrium. Among the five tissue layers of the uterus, the H-score of oxytocin receptors in the deep uterine glands was greater than in the superficial uterine glands ( = 0.023) and the circular muscle layer of the myometrium ( = 0.011), but it did not differ from the epithelial layer of the endometrium ( = 0.428) or the longitudinal muscle layer of the myometrium ( = 0.081). Sows with parity numbers ≥ 6 had a lower oxytocin receptor H-score than those with parity numbers 1 ( < 0.001) and 2-5 ( < 0.001). In conclusion, these data emphasize the notable variations in several reproductive parameters and the levels of oxytocin receptor within the uterus of hyperprolific sows. Across the majority of uterine tissue layers, there was a marked decrease in the H-score of the oxytocin receptor in the older sows.
本研究调查了高产母猪不同胎次的生殖器官大体形态及催产素受体分布情况。选取了32头长白×约克夏杂交母猪的生殖器官，根据胎次分为三组：第1胎（n = 10）、第2 - 5胎（n = 12）和≥6胎（n = 10）。所有母猪均因管理问题被淘汰，且均无生殖系统疾病。对卵巢、子宫及生殖系统其他部分进行了大体形态检查。采用免疫组织化学方法，评估子宫五层组织（子宫内膜上皮、浅腺层、深腺层以及子宫肌层的环形和纵形平滑肌）中催产素受体的水平，并使用H评分进行定量分析。母猪的平均年龄、体重及每窝产仔总数分别为799.8 ± 327.8天、213.2 ± 31.7千克和15.5 ± 4.8头。第1胎母猪的排卵数（19.9 ± 2.4）低于第2 - 5胎母猪（29.7 ± 2.0，P = 0.004）和≥6胎母猪（27.7 ± 2.1，P = 0.022）。第1胎母猪的子宫重量（902.9 ± 112.5克）低于第2 - 5胎母猪（1442.1 ± 111.8克，P = 0.001）和≥6胎母猪（1394.3 ± 125.1克，P = 0.004）。第1胎母猪的子宫长度（277.9 ± 26.1厘米）短于第2 - 5胎母猪（354.6 ± 25.9厘米，P = 0.033），且有短于≥6胎母猪的趋势（346.6 ± 29.0厘米，P = 0.068）。在猪子宫内膜和子宫肌层的不同部位均可检测到催产素受体的免疫定位。在子宫的五层组织中，子宫深腺层催产素受体的H评分高于浅腺层（P = 0.023）和子宫肌层环形肌层（P = 0.011），但与子宫内膜上皮层（P = 0.428）和子宫肌层纵形肌层（P = 0.081）无差异。≥6胎母猪的催产素受体H评分低于第1胎母猪（P < 0.001）和第2 - 5胎母猪（P < 0.001）。总之，这些数据表明高产母猪子宫内的几个生殖参数及催产素受体水平存在显著差异。在大多数子宫组织层中，年龄较大母猪的催产素受体H评分显著降低。
