Liu Jianxin, Yang Danchen Aaron, Qu Haobo, Liu Dandan, Huang Kehe
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Animal Nutritional Health, Nanjing Agricultural University, Nanjing 210095, China.
Animals (Basel). 2024 Jul 4;14(13):1978. doi: 10.3390/ani14131978.
To investigate the effects of on the specific immune response of lactating sows to and the diarrhea rate in suckling piglets, thirty large white sows with similar farrowing dates were randomly divided into two groups: a feedback feeding (i.e., feeding a homogenate of intestinal contents and tissues from -infected piglets to sows; FB) group and a feedback feeding with (FB + BS) group. Serum, colostrum, and intestinal tissues from sows and piglets were collected to assess the immune response and intestinal barrier function at weaning. T and B cells from Peyer's patches (PPs) and mesenteric lymph nodes (MLNs) in lactating mice (with treatments consistent with the sows') were isolated to explore the underlying mechanism. The results showed that, compared with the FB group, the reproductive performance of sows and the growth performance of their offspring were effectively improved in the FB + BS group. Moreover, the levels of IgG/IgA and those of IgG/IgA against in the serum and colostrum of sows in the FB+BS group were increased ( < 0.05). Meanwhile, the ratio of CD4+/CD8+, CD4+CXCR5+PD1+, and B220+IgA+ cells in MLNs and PPs, and the IgA levels in the mammary glands of mice, were also increased in the FB + BS group ( < 0.05). Notably, in suckling piglets in the FB + BS group, the diarrhea rate was decreased ( < 0.05), and the intestinal barrier function and intestinal flora composition at weaning were significantly improved. Overall, these results indicated that feed supplementation combined with feedback feeding in pregnant and lactating sows can reduce diarrhea in suckling piglets by enhancing the maternal immune response against and intestinal barrier function in their offspring, improving survival rates and pre-weaning growth.
为研究[具体物质]对哺乳母猪针对[病原体]的特异性免疫反应及哺乳仔猪腹泻率的影响，将30头分娩日期相近的大白母猪随机分为两组：反馈饲喂组（即给母猪饲喂感染[病原体]仔猪的肠内容物和组织匀浆；FB组）和反馈饲喂加[具体物质]组（FB + BS组）。采集母猪和仔猪的血清、初乳及肠道组织，以评估断奶时的免疫反应和肠道屏障功能。分离哺乳期小鼠（处理与母猪一致）派伊尔氏结（PPs）和肠系膜淋巴结（MLNs）中的T细胞和B细胞，以探究潜在机制。结果显示，与FB组相比，FB + BS组母猪的繁殖性能及其后代的生长性能得到有效改善。此外，FB + BS组母猪血清和初乳中IgG/IgA水平以及针对[病原体]的IgG/IgA水平升高（P < 0.05）。同时，FB + BS组MLNs和PPs中CD4+/CD8+、CD4+CXCR5+PD1+及B220+IgA+细胞的比例以及小鼠乳腺中的IgA水平也升高（P < 0.05）。值得注意的是，FB + BS组哺乳仔猪的腹泻率降低（P < 0.05），断奶时的肠道屏障功能和肠道菌群组成显著改善。总体而言，这些结果表明，在妊娠和哺乳母猪中补充[具体物质]并结合反馈饲喂可通过增强母体对[病原体]的免疫反应及其后代的肠道屏障功能来降低哺乳仔猪腹泻，提高成活率和断奶前生长。
