Cai Jiajun, Qiu Zihao, Liao Jinmei, Li Ansheng, Chen Jiahao, Wu Zehui, Khan Waqar, Sun Binmei, Liu Shaoqun, Zheng Peng
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Soil and Fertilizer Station of Cenxi City, Wuzhou 543200, China.
Foods. 2024 Jul 1;13(13):2091. doi: 10.3390/foods13132091.
Reasonable application of nitrogen fertilizer can improve the yield and quality of tea. This study used Jin Xuan as the tested variety and applied nitrogen fertilizer at rates of 0 kg/ha (N0), 150 kg/ha (N150), 300 kg/ha (N300), and 450 kg/ha (N450) in the summer and autumn seasons to analyze the effects of nitrogen application on the quality components and gene expression of tea leaves. The results showed that the N150 treatment significantly increased total polyphenols (TP), total catechins (TC), and caffeine contents, with the most significant increase observed in the content of six monomers of catechins (EGCG, ECG, EGC, GCG, GC, and EC) in the summer. The N300 treatment significantly increased TP and AA contents in the autumn while decreasing TC content. Additionally, the N300 treatment significantly increased caffeine and theanine contents in the autumn. Notably, the N300 treatment significantly increased both summer and autumn tea yields. Multivariate statistical analysis showed that TPs, AAs, TCs, EGC, and caffeine were key factors affecting the quality of Jin Xuan. Furthermore, the N150 treatment upregulated the expression of the phenylalanine ammonia-lyase () gene, which may increase the accumulation of catechins. In conclusion, it is recommended to apply 150 kg/ha of nitrogen fertilizer in the summer and 300 kg/ha of nitrogen fertilizer in the autumn. This recommendation provides a theoretical basis for improving the quality and yield of tea leaves in summer and autumn.
合理施用氮肥可提高茶叶产量和品质。本研究以金萱为供试品种,在夏秋季节分别施氮量为0 kg/公顷(N0)、150 kg/公顷(N150)、300 kg/公顷(N300)和450 kg/公顷(N450),分析施氮对茶叶品质成分和基因表达的影响。结果表明,N150处理显著提高了总多酚(TP)、总儿茶素(TC)和咖啡因含量,夏季儿茶素六种单体(表没食子儿茶素没食子酸酯、表儿茶素没食子酸酯、表没食子儿茶素、没食子儿茶素没食子酸酯、没食子儿茶素和儿茶素)含量增加最为显著。N300处理显著提高了秋季TP和氨基酸(AA)含量,同时降低了TC含量。此外,N300处理显著提高了秋季咖啡因和茶氨酸含量。值得注意的是,N300处理显著提高了夏秋茶产量。多元统计分析表明,TP、AA、TC、表没食子儿茶素和咖啡因是影响金萱品质的关键因素。此外,N150处理上调了苯丙氨酸解氨酶()基因的表达,这可能增加儿茶素的积累。综上所述,建议夏季施氮量为150 kg/公顷,秋季施氮量为300 kg/公顷。该建议为提高夏秋茶叶品质和产量提供了理论依据。