Guo Qingyan, Peng Jiabao, Zhao Jingjing, Lei Jie, Huang Yukun, Shao Bing
Food Microbiology Key Laboratory of Sichuan Province, School of Food and Bioengineering, Xihua University, Chengdu 610039, China.
Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chengdu 610039, China.
Foods. 2024 Jul 2;13(13):2108. doi: 10.3390/foods13132108.
Broad bean paste (BBP) is a traditional fermented soy food, and its high salt content not only prolongs the fermentation time but also threatens human health. In this study, three BBP-meju with different salt concentrations were prepared, and the effects of varying salinity on fermentation were comprehensively compared. The results showed that salt-reduced fermentation contributed to the accumulation of amino acid nitrogen, reducing sugars, free amino acids, and organic acids. Alcohols, esters, aldehydes, and acids were the main volatile flavor compounds in BBP-meju, and the highest total volatile flavor compounds were found in medium-salt meju. , , , and were the dominant microbial communities during fermentation, and there were also three opportunistic pathogens, , , and , respectively. According to Spearman correlation analysis, , , , and all showed highly significant positive correlations with ≥3 key flavor compounds, which may be the core functional flora. Furthermore, the dominant microbial genera worked synergistically to promote the formation of high-quality flavor compounds and inhibit the production of off-flavors during salt-reduced fermentation. This study provides a theoretical reference for the quality and safety control of low-salt fermented soy foods.
豆瓣酱(BBP)是一种传统发酵豆制品,其高盐含量不仅延长了发酵时间,还威胁人体健康。本研究制备了三种不同盐浓度的豆瓣酱酒曲,并全面比较了不同盐度对发酵的影响。结果表明,低盐发酵有助于氨基酸态氮、还原糖、游离氨基酸和有机酸的积累。醇类、酯类、醛类和酸类是豆瓣酱酒曲中的主要挥发性风味化合物,中盐酒曲中的总挥发性风味化合物含量最高。 、 、 和 是发酵过程中的优势微生物群落,同时还分别存在三种机会致病菌, 、 和 。根据Spearman相关性分析, 、 、 和 与≥3种关键风味化合物均呈极显著正相关,可能是核心功能菌群。此外,优势微生物属协同作用,促进了低盐发酵过程中优质风味化合物的形成,并抑制了异味的产生。本研究为低盐发酵豆制品的质量和安全控制提供了理论参考。