Shao Long, Zhang Biyu, Wu Wei, Cui Gengyan, Liu Mao
School of Metallurgy, Northeastern University, Shenyang 110819, China.
School of Materials Science and Engineering, Changchun University of Science and Technology, Changchun 130012, China.
Nanomaterials (Basel). 2024 Jun 29;14(13):1127. doi: 10.3390/nano14131127.
Highly sensitive and specific biomarker detection is of outstanding importance for the diagnosis and treatment of cancers. Herein, we developed robust photoelectrochemical (PEC) biosensors with low background noise and high sensitivity based on a heterojunction, which can improve semiconductor photoelectric properties by limiting the recombination of photogenerated electron-hole pairs and successfully widening the range of light absorption. Alpha-fetoprotein (AFP) was used as a target model to examine the analytical performances of the designed PEC biosensors. ZnO/CsMnBr heterogeneous film with a uniform porous structure and large surface area enhanced electron transfer and biomolecule immobilization, and significantly increased the photocurrent response. Under the optimal conditions, the designed PEC biosensor exhibited a linear detection range of 0.01-500 ng/mL and a detection limit of 12 pg/mL. In addition, this PEC biosensor performed well when testing human serum samples and exhibited good repeatability, stability over time, and specificity, showing enormous potential for the detection of cancer markers in future biological and clinical research.
高灵敏度和特异性的生物标志物检测对于癌症的诊断和治疗至关重要。在此，我们基于异质结开发了具有低背景噪声和高灵敏度的稳健光电化学（PEC）生物传感器，该异质结可通过限制光生电子-空穴对的复合并成功拓宽光吸收范围来改善半导体光电性能。甲胎蛋白（AFP）用作目标模型来检验所设计的PEC生物传感器的分析性能。具有均匀多孔结构和大表面积的ZnO/CsMnBr异质膜增强了电子转移和生物分子固定，并显著提高了光电流响应。在最佳条件下，所设计 的PEC生物传感器的线性检测范围为0.01-500 ng/mL，检测限为12 pg/mL。此外，该PEC生物传感器在检测人血清样本时表现良好，具有良好的重复性、随时间的稳定性和特异性，在未来的生物学和临床研究中显示出检测癌症标志物的巨大潜力。
