Department of Laboratory Medicine and Pathology, University of Minnesota, MMC 609, 420 Delaware Street, Minneapolis, MN 55455, USA.
Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN 55455, USA.
Int J Mol Sci. 2024 Jun 21;25(13):6831. doi: 10.3390/ijms25136831.
Though the microbiome's impact on immune system homeostasis is well documented, the effect of circulating T cells on the gut microbiome remains unexamined. We analyzed data from 50 healthy volunteers in a pilot trial of aspirin, using immunophenotyping and 16S rRNA sequencing to evaluate the effect of baseline T cells on microbiome changes over 6 weeks. We employed an unsupervised sparse canonical correlation analysis (sCCA) and used multivariable linear regression models to evaluate the association between selected T cell subsets and selected bacterial genera after adjusting for covariates. In the cross-sectional analysis, percentages of naïve CD4+ T cells were positively associated with a relative abundance of , and the percentage of activated CD8+ T cells was inversely associated with . In the longitudinal analysis, the baseline percentages of naïve CD4+ T cells and activated CD4+ T cells were inversely associated with a 6-week change in the relative abundance of and , respectively. The baseline percentage of terminal effector CD4+ T cells was positively associated with the change in . Notably, the microbiome taxa associated with T cell subsets exclusively belonged to the . These findings can guide future experimental studies focusing on the role of T cells in impacting gut microbiome homeostasis.
尽管微生物组对免疫系统稳态的影响已有充分记录，但循环 T 细胞对肠道微生物组的影响仍未被研究。我们分析了 50 名健康志愿者在阿司匹林初步试验中的数据，使用免疫表型和 16S rRNA 测序来评估基线 T 细胞对 6 周内微生物组变化的影响。我们采用无监督稀疏典型相关分析（sCCA）和多变量线性回归模型来评估在调整协变量后选定的 T 细胞亚群与选定细菌属之间的关联。在横断面分析中，幼稚 CD4+T 细胞的百分比与相对丰度呈正相关，而激活的 CD8+T 细胞的百分比与呈负相关。在纵向分析中，基线幼稚 CD4+T 细胞和激活的 CD4+T 细胞的百分比与相对丰度的 6 周变化呈负相关，分别为和。终末效应 CD4+T 细胞的基线百分比与变化呈正相关。值得注意的是，与 T 细胞亚群相关的微生物群分类群仅属于。这些发现可以指导未来专注于 T 细胞在影响肠道微生物组稳态中的作用的实验研究。
