Endocrinology Department, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1. Col. Sección XVI, Mexico City 14080, Mexico.
Research Direction, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1. Col. Sección XVI, Mexico City 14080, Mexico.
Int J Mol Sci. 2024 Jun 27;25(13):7078. doi: 10.3390/ijms25137078.
Type 2 diabetes mellitus (T2DM) is associated with various complications, including diabetic foot, which can lead to significant morbidity and mortality. Non-healing foot ulcers in diabetic patients are a major risk factor for infections and amputations. Despite conventional treatments, which have limited efficacy, there is a need for more effective therapies. MicroRNAs (miRs) are small non-coding RNAs that play a role in gene expression and have been implicated in diabetic wound healing. miR expression was analyzed through RT-qPCR in 41 diabetic foot Mexican patients and 50 controls. Diabetic foot patients showed significant increases in plasma levels of miR-17-5p ( = 0.001), miR-191-5p ( = 0.001), let-7e-5p ( = 0.001), and miR-33a-5p ( = 0.005) when compared to controls. Elevated levels of miR-17, miR-191, and miR-121 correlated with higher glucose levels in patients with diabetic foot ulcers (r = 0.30, = 0.004; r = 0.25, = 0.01; and r = 0.21, = 0.05, respectively). Levels of miR-17 showed the highest diagnostic potential (AUC 0.903, = 0.0001). These findings underscore the possible role of these miRs in developing diabetes complications. Our study suggests that high miR-17, miR-191, and miR-121 expression is strongly associated with higher glucose levels and the development of diabetic foot ulcers.
2 型糖尿病（T2DM）与多种并发症相关，包括糖尿病足，可导致较高的发病率和死亡率。糖尿病患者的难愈合足部溃疡是感染和截肢的主要危险因素。尽管传统治疗方法疗效有限，但仍需要更有效的治疗方法。microRNAs（miRs）是一种小的非编码 RNA，在基因表达中起作用，并与糖尿病伤口愈合有关。通过 RT-qPCR 分析了 41 例墨西哥糖尿病足患者和 50 例对照者的 miR 表达。与对照组相比，糖尿病足患者的血浆 miR-17-5p（ = 0.001）、miR-191-5p（ = 0.001）、let-7e-5p（ = 0.001）和 miR-33a-5p（ = 0.005）水平显著升高。糖尿病足溃疡患者 miR-17、miR-191 和 miR-121 水平升高与血糖水平升高相关（r = 0.30， = 0.004；r = 0.25， = 0.01；r = 0.21， = 0.05）。miR-17 水平具有最高的诊断潜力（AUC 0.903， = 0.0001）。这些发现强调了这些 miRs 在糖尿病并发症发展中的可能作用。我们的研究表明，miR-17、miR-191 和 miR-121 的高表达与高血糖水平和糖尿病足溃疡的发生密切相关。
