State Key Laboratory of Efficient Production of Forest Resources, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.
Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520, China.
Int J Mol Sci. 2024 Jun 29;25(13):7214. doi: 10.3390/ijms25137214.
The toxic metal cadmium (Cd) poses a serious threat to plant growth and human health. calcium-dependent protein kinase 21 (CPK21) has previously been shown to attenuate Cd toxicity by reducing Cd accumulation, enhancing antioxidant defense and improving water balance in transgenic Arabidopsis. Here, we confirmed a protein-protein interaction between PeCPK21 and Arabidopsis nuclear transcription factor YC3 (AtNF-YC3) by yeast two-hybrid and bimolecular fluorescence complementation assays. was induced by Cd and strongly expressed in -overexpressed plants. Overexpression of in Arabidopsis reduced the Cd inhibition of root length, fresh weight and membrane stability under Cd stress conditions (100 µM, 7 d), suggesting that AtNF-YC3 appears to contribute to the improvement of Cd stress tolerance. AtNF-YC3 improved Cd tolerance by limiting Cd uptake and accumulation, activating antioxidant enzymes and reducing hydrogen peroxide (HO) production under Cd stress. We conclude that PeCPK21 interacts with AtNF-YC3 to limit Cd accumulation and enhance the reactive oxygen species (ROS) scavenging system and thereby positively regulate plant adaptation to Cd environments. This study highlights the interaction between PeCPK21 and AtNF-YC3 under Cd stress conditions, which can be utilized to improve Cd tolerance in higher plants.
有毒金属镉（Cd）对植物生长和人类健康构成严重威胁。先前已经证明钙依赖性蛋白激酶 21（CPK21）通过减少 Cd 积累、增强抗氧化防御和改善转基因拟南芥的水平衡来减轻 Cd 毒性。在这里，我们通过酵母双杂交和双分子荧光互补实验证实了 PeCPK21 与拟南芥核转录因子 YC3（AtNF-YC3）之间的蛋白-蛋白相互作用。在 Cd 诱导下，在 -过表达植物中强烈表达。拟南芥中 的过表达在 Cd 胁迫条件下（100 µM，7 d）降低了 Cd 对根长、鲜重和膜稳定性的抑制作用，表明 AtNF-YC3 似乎有助于提高 Cd 胁迫耐受性。AtNF-YC3 通过限制 Cd 吸收和积累、激活抗氧化酶和减少 Cd 胁迫下的过氧化氢（HO）产生来提高 Cd 耐受性。我们得出结论，PeCPK21 与 AtNF-YC3 相互作用以限制 Cd 积累并增强活性氧（ROS）清除系统，从而正向调节植物对 Cd 环境的适应。本研究强调了 Cd 胁迫条件下 PeCPK21 和 AtNF-YC3 之间的相互作用，可用于提高高等植物的 Cd 耐受性。
