Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Int J Mol Sci. 2024 Jul 3;25(13):7318. doi: 10.3390/ijms25137318.
() is a gene encoding an enzyme related to the last step of juvenile hormone biosynthesis. cDNA has a total length of 1695 bp and an open reading frame (ORF) length of 1482 bp, encoding 493 amino acids. Sequence analysis showed that its amino acid sequence has a PPGP hinge, an FGCG structural domain, and other structural domains specific to the P450 family of enzymes. was most highly expressed in the hepatopancreas, followed by the ovary and gill, weakly expressed in heart and muscle tissue, and barely expressed in the eyestalk and cranial ganglion. expression remained stable during the larval period, during which it mainly played a critical role in gonadal differentiation. Expression in the ovary was positively correlated and expression in the hepatopancreas was negatively correlated with ovarian development. In situ hybridization (ISH) showed that the signal was expressed in the oocyte, nucleus, cell membrane and follicular cells, and the intensity of expression was strongest at stage O-IV. The knockdown of resulted in a significantly lower gonadosomatic index and percentage of ovaries past stage O-III compared to the control group. However, no differences were found in the cumulative frequency of molting between the experimental and control groups. Moreover, the analysis of ovarian tissue sections at the end of the experiment showed differences between groups in development speed but not in subcellular structure. These results demonstrate that promotes the ovarian development of adults but has no effect on molting.
()是一个编码与保幼激素生物合成最后一步相关的酶的基因。cDNA 全长 1695bp，开放阅读框（ORF）长 1482bp，编码 493 个氨基酸。序列分析表明，其氨基酸序列具有 PPGP 铰链、FGCG 结构域和其他 P450 家族酶特有的结构域。在肝胰腺中表达量最高，其次是卵巢和鳃，在心和肌肉组织中表达较弱，在眼柄和脑神经节中几乎不表达。在幼虫期表达保持稳定，在此期间，它主要在性腺分化中发挥关键作用。在卵巢中的表达与卵巢发育呈正相关，在肝胰腺中的表达与卵巢发育呈负相关。原位杂交（ISH）显示信号在卵母细胞、细胞核、细胞膜和滤泡细胞中表达，在 O-IV 期表达最强。与对照组相比，敲低后的 导致性腺指数和 O-III 期以上卵巢的比例明显降低。然而，实验组和对照组在蜕皮累积频率上没有差异。此外，实验结束时对卵巢组织切片的分析表明，两组在发育速度上存在差异，但在亚细胞结构上没有差异。这些结果表明， 促进了 的卵巢发育，但对蜕皮没有影响。
