Ufa Institute of Biology, Ufa Federal Research Centre, the Russian Academy of Sciences, Prospekt Oktyabrya, 69, 450054 Ufa, Russia.
M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
Int J Mol Sci. 2024 Jul 8;25(13):7486. doi: 10.3390/ijms25137486.
The adaption of plants to stressful environments depends on long-distance responses in plant organs, which themselves are remote from sites of perception of external stimuli. Jasmonic acid (JA) and its derivatives are known to be involved in plants' adaptation to salinity. However, to our knowledge, the transport of JAs from roots to shoots has not been studied in relation to the responses of shoots to root salt treatment. We detected a salt-induced increase in the content of JAs in the roots, xylem sap, and leaves of pea plants related to changes in transpiration. Similarities between the localization of JA and lipid transfer proteins (LTPs) around vascular tissues were detected with immunohistochemistry, while immunoblotting revealed the presence of LTPs in the xylem sap of pea plants and its increase with salinity. Furthermore, we compared the effects of exogenous MeJA and salt treatment on the accumulation of JAs in leaves and their impact on transpiration. Our results indicate that salt-induced changes in JA concentrations in roots and xylem sap are the source of accumulation of these hormones in leaves leading to associated changes in transpiration. Furthermore, they suggest the possible involvement of LTPs in the loading/unloading of JAs into/from the xylem and its xylem transport.
植物对胁迫环境的适应取决于植物器官的长距离反应，而这些器官本身远离外部刺激感知的部位。茉莉酸（JA）及其衍生物被认为参与了植物对盐度的适应。然而，据我们所知，JA 从根部向地上部的运输与地上部对根部盐处理的反应之间的关系尚未得到研究。我们检测到豌豆植株根系、木质部汁液和叶片中 JA 的含量随着蒸腾作用的变化而增加，这与盐胁迫有关。免疫组织化学检测到 JA 与围绕维管束组织的脂转移蛋白（LTP）之间的定位相似性，而免疫印迹显示 LTP 存在于豌豆植株的木质部汁液中，并且随着盐度的增加而增加。此外，我们比较了外源 MeJA 和盐处理对叶片中 JAs 积累的影响及其对蒸腾作用的影响。我们的结果表明，根部和木质部汁液中 JA 浓度的盐诱导变化是这些激素在叶片中积累的来源，导致蒸腾作用的相关变化。此外，它们还表明 LTPs 可能参与了 JA 从木质部加载/卸载以及木质部运输。
