用于检测马布特罗（一种气管中的选择性β2-肾上腺素能兴奋剂）的酶免疫测定法。

Enzyme immunoassay for mabuterol, a selective beta 2-adrenergic stimulant in the trachea.

作者信息

Yamamoto I, Matsuura E, Horiba M, Akima K, Nomura K, Aizawa T

出版信息

J Immunoassay. 1985;6(3):261-76. doi: 10.1080/01971528508063033.

DOI:10.1080/01971528508063033

Abstract

A sensitive double antibody and heterologous enzyme immunoassay for mabuterol was established. For competitive reactions, antibody raised against diazotized mabuterol-human serum albumin was incubated with a mixture of diazotized mabuterol analog (RC-1) labeled with beta-D-galactosidase and standard or sample. Free and antibody-bound enzyme hapten were separated using anti-rabbit IgG immobilized on polystyrene balls. Activity of the enzyme on the solid phase was fluorometrically determined. The present immunoassay allows detection of 0.5 to 100 pg/tube of mabuterol. Pharmacokinetic behavior of this agent in human plasma and urine was studied after a single oral administration (50 micrograms). The maximum level was achieved after 2-3 hrs with approximately 280 pg mabuterol /ml of plasma and the half life of mabuterol was estimated to be 19.5 hrs. Cumulative amount of mabuterol in the first 72 hrs urine was 64.3 +/- 13.2% of the administered dose.

摘要

建立了一种灵敏的马布特罗双抗体及异源酶免疫分析法。对于竞争性反应，将针对重氮化马布特罗-人血清白蛋白产生的抗体与用β-D-半乳糖苷酶标记的重氮化马布特罗类似物（RC-1）及标准品或样品的混合物一起孵育。使用固定在聚苯乙烯球上的抗兔IgG分离游离的和与抗体结合的酶半抗原。用荧光法测定固相上酶的活性。本免疫分析法可检测出马布特罗0.5至100 pg/管。单次口服给药（50微克）后，研究了该药物在人血浆和尿液中的药代动力学行为。2至3小时后达到最高水平，血浆中马布特罗约为280 pg/ml，马布特罗的半衰期估计为19.5小时。给药后72小时内，尿液中马布特罗的累积量为给药剂量的64.3±13.2%。