Farina Antonella, Viggiani Valentina, Cortese Francesca, Moretti Marta, Tartaglione Sara, Angeloni Antonio, Anastasi Emanuela
Department of Experimental Medicine, "La Sapienza" University of Rome, V. Le Regina Elena 324, 00161 Rome, Italy.
Cancers (Basel). 2024 Jun 27;16(13):2362. doi: 10.3390/cancers16132362.
"Background/Aim": the current inability to diagnose Pancreatic Cancer Adenocarcinoma (PDAC) at an early stage strongly influences therapeutic strategies. Protein Induced by Vitamin K Absence (PIVKA II) showed an accurate diagnostic performance for PDAC. Since circulating PIVKA II has been recently associated with pancreatic origin cells with Vimentin, an epithelial-to-mesenchymal transition (EMT) early activation marker, the aim of this study was to investigate in vivo the combination between the two proteins. "Materials and Methods": we assayed the presence of PIVKA II and Vimentin proteins by using different diagnostic methods. A total of 20 PDAC patients and 10 healthy donors were tested by Western Blot analysis; 74 PDAC patient and 46 healthy donors were assayed by ECLIA and Elisa. "Results": Western Blot analysis showed the concomitant expression of PIVKA II and Vimentin in PDAC patient sera. Immunometric assay performed on a larger cohort of patients demonstrated that 72% of PIVKA II-positive PDAC patients were Vimentin-positive. Additionally, in a group of PDAC patients with PIVKA II levels ≥2070 ng/mL, the percentage of Vimentin-positive subjects reached 84%. "Conclusion": the association between PIVKA II protein and the EMT suggests that this molecule could be considered a marker of the acquisition of an aggressive phenotype.
“背景/目的”：目前无法在早期诊断出胰腺导管腺癌（PDAC），这对治疗策略有很大影响。维生素K缺乏诱导蛋白（PIVKA II）对PDAC显示出准确的诊断性能。由于循环中的PIVKA II最近与具有波形蛋白（一种上皮-间质转化（EMT）早期激活标志物）的胰腺起源细胞相关，本研究的目的是在体内研究这两种蛋白之间的联合情况。“材料与方法”：我们使用不同的诊断方法检测PIVKA II和波形蛋白的存在。通过蛋白质印迹分析对20例PDAC患者和10名健康供体进行检测；通过电化学发光免疫分析（ECLIA）和酶联免疫吸附测定（ELISA）对74例PDAC患者和46名健康供体进行检测。“结果”：蛋白质印迹分析显示PDAC患者血清中PIVKA II和波形蛋白同时表达。对更大患者队列进行的免疫测定表明，72%的PIVKA II阳性PDAC患者波形蛋白呈阳性。此外，在一组PIVKA II水平≥2070 ng/mL的PDAC患者中，波形蛋白阳性受试者的比例达到84%。“结论”：PIVKA II蛋白与EMT之间的关联表明，该分子可被视为侵袭性表型获得的标志物。
