Zhang Jie, Tang Shen, Li Yanhong, Zhang Jing, Peng Yuqing, Peng JingJing, Cen Yu, Shi Peizhu
College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China.
College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China; The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control, Guilin University of Technology, Guilin 541004, China.
J Hazard Mater. 2024 Sep 5;476:135162. doi: 10.1016/j.jhazmat.2024.135162. Epub 2024 Jul 10.
Iron oxide @ biochar (FeO/C) promotes bacterial growth and facilitates electron transfer, thereby effectively promoting malathion degradation by Shewanella oneidensis MR-1 (S. oneidensis MR-1). This study elucidated the underlying mechanism of FeO/C-enhanced malathion degradation by S. oneidensis MR-1 through a combination of metabolomics and proteomics analysis. The kinetic fitting results from the degradation experiment indicated that 0.1 g/L FeO/C exerted the most significant enhancement effect on malathion degradation by S. oneidensis MR-1. Observations from Scanning Electron Microscopy and Laser Scanning Confocal Microscopy, along with physiological and biochemical analysis, showed that FeO/C enhanced the growth and oxidative response of S. oneidensis MR-1 under malathion stress. In addition, metabolomics and proteomics analysis revealed an increase in certain electron transfer related metabolites, such as coenzymes, and the upregulation of proteins, including coenzyme A, sdhD, and petC. Overall, spectroscopic analysis suggested that Fe, which was reduced from Fe by S. oneidensis MR-1 in FeO/C, promoted electron transfer in S. oneidensis MR-1 to enhance the degradation of malathion. This study offers enhanced strategies for efficient removal of malathion contaminants.
氧化铁@生物炭（FeO/C）促进细菌生长并促进电子转移，从而有效促进嗜水气单胞菌MR-1（S. oneidensis MR-1）对马拉硫磷的降解。本研究通过代谢组学和蛋白质组学分析相结合的方法，阐明了FeO/C增强S. oneidensis MR-1对马拉硫磷降解的潜在机制。降解实验的动力学拟合结果表明，0.1 g/L FeO/C对S. oneidensis MR-1降解马拉硫磷的增强作用最为显著。扫描电子显微镜和激光扫描共聚焦显微镜的观察结果，以及生理生化分析表明，FeO/C增强了S. oneidensis MR-1在马拉硫磷胁迫下的生长和氧化反应。此外，代谢组学和蛋白质组学分析显示，某些与电子转移相关的代谢物（如辅酶）增加，以及包括辅酶A、sdhD和petC在内的蛋白质上调。总体而言，光谱分析表明，FeO/C中的Fe被S. oneidensis MR-1还原为Fe，促进了S. oneidensis MR-1中的电子转移，从而增强了马拉硫磷的降解。本研究为高效去除马拉硫磷污染物提供了强化策略。
