Hartskeerl R, Zuidweg E, van Geffen M, Hoekstra W
J Gen Microbiol. 1985 Jun;131(6):1305-11. doi: 10.1099/00221287-131-6-1305.
The exclusion relationship between the IncI plasmids R144, R64 and ColIb was studied in such a way that incompatibility interference was avoided. Genetic crosses with an R144-derived Hfr donor, crosses with recipient strains carrying R144-derived exclusion genes on a recombinant plasmid compatible with R144, and measurement of transmission frequencies of a recombinant plasmid compatible with IncI plasmids after mobilization by R144 revealed that R144, R64 and ColIb belong to one exclusion group.
对IncI质粒R144、R64和ColIb之间的排斥关系进行了研究,以避免不相容干扰。用R144衍生的Hfr供体进行遗传杂交,用在与R144相容的重组质粒上携带R144衍生排斥基因的受体菌株进行杂交,并在R144动员后测量与IncI质粒相容的重组质粒的传递频率,结果表明R144、R64和ColIb属于一个排斥组。