Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung, 91201, Taiwan.
Department of Physics, Kasetsart University, 50 Ngamwongwan Rd, Lat Yao, Chatuchak, Bangkok, 10900, Thailand.
Anal Biochem. 2024 Nov;694:115615. doi: 10.1016/j.ab.2024.115615. Epub 2024 Jul 11.
With the rise in extreme weather due to global warming, coupled with globalization facilitating the spread of infectious diseases, there's a pressing need for portable testing platforms offering simplicity, low cost, and remote transmission, particularly beneficial in resource-limited and non-urban areas. We have developed a portable device using loop-mediated isothermal amplification (LAMP) with spectrometric detection to identify Salmonella Typhimurium DNA. The device utilizes the LinkIt 7697 microcontroller and a microspectrometer to capture and transmit spectral signals in real-time, allowing for improved monitoring and analysis of the reaction progress. We built a hand-held box containing a microspectrometer, thermoelectric cooler, ultraviolet LED, disposable reaction tube, and homemade thermal module, all powered by rechargeable batteries. Additionally, we conducted thorough experiments to ensure temperature accuracy within 1 °C under thermal control, developed a heating module with a LinkIt 7697 IoT development board to heat the DNA mixture to the reaction temperature within 3 min, and integrated foam insulation and a 3D-printed frame to enhance the device's thermal stability. We successfully demonstrated the amplification of Salmonella Typhimurium DNA with an impressive sensitivity of 2.83 × 10 ng/μL. A remote webpage interface allows for monitoring the temperature and fluorescence during the LAMP process, improving usability. This portable LAMP device with real-time detection offers a cost-effective solution for detecting Salmonella Typhimurium in food products. Its unique design and capabilities make it a promising tool for ensuring food safety.
随着全球变暖导致极端天气的增加，加上全球化促进了传染病的传播，因此迫切需要一种便携式检测平台，这种平台应该具有简单、低成本和远程传输的特点，特别有益于资源有限和非城市地区。我们开发了一种使用环介导等温扩增（LAMP）和分光光度检测来识别鼠伤寒沙门氏菌 DNA 的便携式设备。该设备使用 LinkIt 7697 微控制器和微分光光度计实时捕获和传输光谱信号，从而可以更好地监测和分析反应进度。我们制作了一个包含微分光光度计、热电冷却器、紫外 LED、一次性反应管和自制热模块的手提箱，所有这些都由可充电电池供电。此外，我们进行了彻底的实验，以确保在热控制下温度精度在 1°C 以内，开发了一个带有 LinkIt 7697 IoT 开发板的加热模块，可在 3 分钟内将 DNA 混合物加热至反应温度，并集成了泡沫隔热和 3D 打印框架以增强设备的热稳定性。我们成功地展示了对鼠伤寒沙门氏菌 DNA 的扩增，其灵敏度达到了 2.83×10 ng/μL。远程网页界面允许在 LAMP 过程中监测温度和荧光，提高了可用性。这种具有实时检测功能的便携式 LAMP 设备为检测食品中的鼠伤寒沙门氏菌提供了一种具有成本效益的解决方案。其独特的设计和功能使其成为确保食品安全的有前途的工具。
