Chen Lu, Wang Yirong, Liu Hongchang, Zhou Yuhang, Nie Zhenyuan, Xia Jinlan, Shu Wensheng
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China.
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; Key Lab of Biometallurgy of Ministry of Education of China, Central South University, Changsha 410083, China.
J Environ Sci (China). 2025 Jan;147:342-358. doi: 10.1016/j.jes.2023.12.006. Epub 2023 Dec 16.
Secondary iron-sulfate minerals such as jarosite, which are easily formed in acid mine drainage, play an important role in controlling metal mobility. In this work, the typical iron-oxidizing bacterium Acidithiobacillus ferrooxidans ATCC 23270 was selected to synthesize jarosite in the presence of antimony ions, during which the solution behavior, synthetic product composition, and bacterial metabolism were studied. The results show that in the presence of Sb(V), Fe was rapidly oxidized to Fe by A. ferrooxidans and Sb(V) had no obvious effect on the biooxidation of Fe under the current experimental conditions. The presence of Sb(III) inhibited bacterial growth and Fe oxidation. For the group with Sb(III), products with amorphous phases were formed 72 hr later, which were mainly ferrous sulfate and pentavalent antimony oxide, and the amorphous precursor was finally transformed into a more stable crystal phase. For the group with Sb(V), the morphology and structure of jarosite were changed in comparison with those without Sb. The biomineralization process was accompanied by the removal of 94% Sb(V) to form jarosite containing the Fe-Sb-O complex. Comparative transcriptome analysis shows differential effects of Sb(III) and Sb(V) on bacterial metabolism. The expression levels of functional genes related to cell components were much more downregulated for the group with Sb(III) but much more regulated for that with Sb(V). Notably, cytochrome c and nitrogen fixation-relevant genes for the A.f_Fe_Sb(III) group were enhanced significantly, indicating their role in Sb(III) resistance. This study is of great value for the development of antimony pollution control and remediation technology.
次生硫酸铁矿物如黄钾铁矾，很容易在酸性矿山排水中形成，在控制金属迁移率方面发挥着重要作用。在这项工作中，选择典型的铁氧化细菌嗜酸氧化亚铁硫杆菌ATCC 23270在锑离子存在的情况下合成黄钾铁矾，在此期间研究了溶液行为、合成产物组成和细菌代谢。结果表明，在Sb(V)存在的情况下，嗜酸氧化亚铁硫杆菌将Fe迅速氧化为Fe，并且在当前实验条件下Sb(V)对Fe的生物氧化没有明显影响。Sb(III)的存在抑制了细菌生长和Fe氧化。对于含有Sb(III)的组，72小时后形成了具有非晶相的产物，主要是硫酸亚铁和五价氧化锑，并且非晶态前体最终转变为更稳定的晶相。对于含有Sb(V)的组，与不含Sb的情况相比，黄钾铁矾的形态和结构发生了变化。生物矿化过程伴随着94%的Sb(V)被去除，形成了含有Fe-Sb-O络合物的黄钾铁矾。比较转录组分析表明Sb(III)和Sb(V)对细菌代谢有不同影响。对于含有Sb(III)的组，与细胞成分相关的功能基因的表达水平下调得多得多，但对于含有Sb(V)的组则上调得多得多。值得注意的是，嗜酸氧化亚铁硫杆菌Fe-Sb(III)组的细胞色素c和与固氮相关的基因显著增强，表明它们在抗Sb(III)中的作用。这项研究对于锑污染控制和修复技术的发展具有重要价值。
