Fang Linfa, Lakshmanan Prakash, Su Xiaoxuan, Shi Yujia, Chen Zheng, Zhang Yu, Sun Wei, Wu Junxi, Xiao Ran, Chen Xinping
Interdisciplinary Research Center for Agriculture Green Development in Yangtze River Basin, College of Resources and Environment, Southwest University, Chongqing 400715, China.
Interdisciplinary Research Center for Agriculture Green Development in Yangtze River Basin, College of Resources and Environment, Southwest University, Chongqing 400715, China; Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs; Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St Lucia 4067, QLD, Australia.
J Environ Sci (China). 2025 Jan;147:498-511. doi: 10.1016/j.jes.2023.10.021. Epub 2023 Oct 28.
The land application of livestock manure has been widely acknowledged as a beneficial approach for nutrient recycling and environmental protection. However, the impact of residual antibiotics, a common contaminant of manure, on the degradation of organic compounds and nutrient release in Eutric Regosol is not well understood. Here, we studied, how oxytetracycline (OTC) and ciprofloxacin (CIP) affect the decomposition, microbial community structure, extracellular enzyme activities and nutrient release from cattle and pig manure using litterbag incubation experiments. Results showed that OTC and CIP greatly inhibited livestock manure decomposition, causing a decreased rate of carbon (28%-87%), nitrogen (15%-44%) and phosphorus (26%-43%) release. The relative abundance of gram-negative (G-) bacteria was reduced by 4.0%-13% while fungi increased by 7.0%-71% during a 28-day incubation period. Co-occurrence network analysis showed that antibiotic exposure disrupted microbial interactions, particularly among G- bacteria, G+ bacteria, and actinomycetes. These changes in microbial community structure and function resulted in decreased activity of urease, β-1,4-N-acetyl-glucosaminidase, alkaline protease, chitinase, and catalase, causing reduced decomposition and nutrient release in cattle and pig manures. These findings advance our understanding of decomposition and nutrient recycling from manure-contaminated antibiotics, which will help facilitate sustainable agricultural production and soil carbon sequestration.
畜禽粪便的土地施用已被广泛认为是一种有益的养分循环和环境保护方法。然而，粪便中常见污染物残留抗生素对中性普通土中有机化合物降解和养分释放的影响尚不清楚。在此，我们通过凋落物袋培养实验研究了土霉素（OTC）和环丙沙星（CIP）如何影响牛和猪粪便的分解、微生物群落结构、胞外酶活性和养分释放。结果表明，OTC和CIP极大地抑制了畜禽粪便的分解，导致碳（28%-87%）、氮（15%-44%）和磷（26%-43%）的释放速率降低。在28天的培养期内，革兰氏阴性（G-）菌的相对丰度降低了4.0%-13%，而真菌增加了7.0%-71%。共现网络分析表明，抗生素暴露破坏了微生物相互作用，特别是在G-菌、G+菌和放线菌之间。微生物群落结构和功能的这些变化导致脲酶、β-1,4-N-乙酰葡糖胺糖苷酶、碱性蛋白酶、几丁质酶和过氧化氢酶的活性降低，从而导致牛和猪粪便的分解和养分释放减少。这些发现增进了我们对受粪便污染抗生素的分解和养分循环的理解，这将有助于促进可持续农业生产和土壤碳固存。
